RNDr. Veronika Huntošová, PhD.

Current positions: 

  • researcher – head of Confocal microscopy and Time resolved microscopy laboratories

Education:

2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics

2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics

2008 Safarik University Kosice – rigorous exam RNDr. in Physic

2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et Marie Curie ED 387 Sorbonne Universites

2011 – 2013 Post-Doc fellow, Ecole Polytechnique Federale de Lausanne (Switzerland)

Research interests:

  • Monitoring of cell/tissue oxygenation and oxidative stress by steady state and time-resolved microscopy;
  • Monitoring of cell signalling related to cell survival, utophagy and apoptosis in cancer;
  • Development of nanoscale transport systems for cancer targeting;
  • Application of avian chorioallantoic membrane model for photodiagnosis, photodynamic therapy, and tumor targeting;

Education:

2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics
2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics
2008 Safarik University Kosice – rigorous exam RNDr. in Physics

2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et Marie Curie ED 387 Sorbonne Universites

Professional positions:

2010 – 2011 Research fellow, Department of Biophysics, Safarik University Kosice
2011 – 2013 Post-Doc fellow, Ecole Polytechnique Federale de Lausanne (Switzerland)
2013 – Research fellow, Center for Interdisciplinary Biosciences, Technology and innovation park, Safarik University Kosice

Research training:

2005 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2006 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2008 Pierre et Marie Curie University in Paris 6 (France) (3 months)
2010 EBSA Biophysics Course in Bordeaux-Arcachon, France (1 week)
2012 ESP Photobiology School in Brixen/Bressanone, Italy (1 week)
2013-2016 Swiss federal institute of technology (EPFL) in Lausanne (Switzerland) (4 x 1 month)
2013-2016 Pierre et Marie Curie University in Paris 6 (France) (3x 1 month)

2023 Erasmus+ staff mobility for training – stay in the laboratory of Dr. Vamosi at Dept. of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, Hungary (7 days)

2023 Erasmus+ staff mobility for training – stay in the laboratory of assoc. prof. Unterhuber at Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Austria (5 days)

Methodology:

UV-VIS absorption spectroscopy, fluorescence spectroscopy, time-resolved spectroscopy/microscopy, fluorescence and fluorescence confocal-microscopy, stopped-flow spectroscopy, flow-cytometry analysis of cellular processes, cells viability tests, isolation of cellular structures, immunofluorescence, western blotting, cultivation of cells cultures, incubation and preparation of quail, turkey and chick’s chorioallantoic membranes (CAM) in vivo, tests of phototoxicity in vivo in the CAM model.

Publications:

Number of articles in journals registered in WoS and SCOPUS: 53
Number of SCI citations (self-citations excluded): 397
H-index: 16

Membership in scientific societies:

2021-present Slovak Society for Biochemistry and Molecular Biology

2017-present Czechoslovak Microscopy society and Microscopy society, from 2023 a member of a board
2015-present Biophysical Society-USA and Europe
2015-present Slovak Biophysical Society (SKBS), from 2017-2020 a member of a SKBS board, 2020-secretary of SKBS

2012-2013 International society on oxygen transport to tissue (ISOTT)

2006-present European Society for Photobiology (ESP)

2023 – present Editorial Advisory Board in Photodiagnosis and Photodynamic Therapy

2023 – Guest Editor in Frontiers in Cell and Development, Reviews and Advances in Cutting Edge Microscopy and Imaging Techniques in Membrane Trafficking and Organellar Dynamics

2022-2023 Guest Editor in Frontiers in Cell and Development, Molecular and Cellular Pathology, Research Topic: Advanced Imaging and Its Application in Biology and Medicine (2022)

2022-2023 Special Issue Editor in Pharmaceuticals, Special Issue: Fluorescence Approaches in Drug Delivery

Teaching:

ÚFV/FChFB/14 – Fotochémia a fotobiológia

ÚFV/CB/14 – Bunková biológia

ÚFV/EMBF3/18 – Experimentálne metódy biofyziky III

ÚFV/PEMBF3/18 – Praktikum k experimentálnym metódam biofyziky III

ÚFV/FCH1/02 –             Fyzikálna chémia pre biologické vedy

Supervision:

Thesis supervisions (4+5):

2023

  • Pevna: Mechanism of photodynamic therapy in 3D spheroids. Supervisor (in progress)
  • Olejarova: Monitoring of cell signalling in photobiomodulation and photodynamic therapy. Supervisor (in progress)
  • Timulakova: Development of nanoparticle transport system for drug delivery and bioimaging. Supervisor (in progress)
  • Migasova: Porous coordination polymers for drug transport. Co-supervisor (in progress)
  • Varcholova: Raman and SERS spectroscopy and imaging of (living) cells: routine use of Raman microscopy to characterize structural and morphological changes. Co-supervisor (in progress)

2022

  • Tomkova: Role of inter-organelle communication in cell function. Co-supervisor (completed)
  • Lenkavska: Development of transport systems designed for targeted transport of hydrophobic drugs into cancer cells. Supervisor (completed)

2017

  • Blascakova: Development of drug nanodelivery vehicles based on lipids, lipoproteins and saponins. Co-supervisor (completed)

2016

  • Verebova: Investigation of interactions between biological macromolecules and photoactive molecules. Co-supervisor (completed)

Master’s thesis supervisions (3):

2021

  • Jadudova: Investigation of nanosensors for monitoring of tissues and cells oxygenation and the level of oxidative stress in cancer cells (completed)

2020

  • Ambrozova: The study of Darpins biodistribution and their influence on cancer cells viability in vitro and ex ovo: the quail chorioallantoic membrane model (completed)

2015

  • Jenikova: Influence of environment and photoproducts on PpIX luminescence detection(completed)

Bachelor’s thesis supervisions (5):

2023

  • Zavacká: Interaction of proteins containing a domain sensitive to light-oxygen-voltage with cancer cells (in progress)

2022

  • Palková: Investigation of metabolic changes in 3D spheorids of cancer cells using confocal fluorescence microscopy (completed)

2021

  • Lukacova: The study of the luminescence oxygen sensitive probes (completed)

2019

  • Jadudova: The study of CeO2 nanoparticles uptake and cytotoxic effect on cancer cells (completed)
  • Abdessamad: Monitoring of oxidative damage in cells during photodynamic therapy(completed)

Secondary school student scientific work supervisions:

2022

  • Grossrubatscher: Application of hypericin in photodynamic therapy.

2020

  • Burdova: Využitie kombinácie acidifikácie extracelulárneho pH nádorových buniek a účinkov rottlerinu ako jedného zo spôsobov protinádorovej terapie.Achievement: AMAVET Diploma, and certificate to participate at EU contest for young scientist EUCYS in Salamanca, Spain 2021)
  • Burdova: Využitie kombinácie acidifikácie extracelulárneho pH nádorových buniek a účinkov rottlerinu ako jedného zo spôsobov protinádorovej terapie. 1stplace at the regional secondary school student’s competition

2019

  • Nagyova, K. Mitrova a G. Mitrova: Interakcia hypericínu s kvasinkami.

2018

  • Motesicka: Influence of PKCδ regulators on photodynamic therapy efficacy.EU contest for young scientist EUCYS in Dublin, Ireland 2018. Achievement: EUCYS 2018 prize winner entitled her to attend a conference at EMBL, Heidelberg); interview in press SK
  • Motesicka: Prírodné prvky ako liek alebo prevencia proti rakovine. 1stplace at the regional secondary school student’s competition
  • Mitrova a B. Nagyova: Využitie ľubovníka bodkovaného vo fotodynamickej terapii.1st place at the regional secondary school student’s competition.
  • Korossyova a M. Adamjak: Charakterizácia nádorových sféroidov

2017

 Invited lectures:

  • Huntosova V (invited speaker): Time-resolved detection of oxidative stress level in cancer cells, Regional Biophysics Conference 22-26 August (2022) Pecs, Hungary
  • Huntosova V.: Detection of singlet oxygen using time-resolved techniques. LLE TReT workshop on Time-resolved techniques, 20-22thJune 2018, BioCeV, Vestec, Czech Republic
  • Huntosova V.: Mitochondria and lysosomes movement during photodynamic action in living cells. Andor Academy, 7-9th November 2017, Center of Biosciences SAS in Bratislava, Slovakia
  • Huntosova V.: Reorganization and dynamics of subcellular organelles during anticancer treatment. Nanoscopy studies vol. 1 – Teoretical and practical sessions with Leica STED 3X, 19-21st September 2017, Center of Biosciences SAS in Bratislava, Slovakia
  • Huntosova V., Sokolova S., Lenkavska L., Miskovsky P.: Time-resolved visualization of oxidative stress during apoptosis in cancer cells by fluorescent probes. 9-10th May 2017, Bratislava, Slovakia
  • Huntosova V.: Drug delivery systems and oxygen detection in photodynamic therapy and photodyagnostics. 7thSlovak Biophysical Symposium, Novy Smokovec, Slovakia, 6-8. April 2016
  • Huntosova V.: Photons in a way to study biostructures, ALUMNI SPACE, P.J. Safarik University in Kosice, Slovakia, 6. February 2015

 Popularization lectures:

  • Huntosova V.: A light helps to heal: Photodynamic Therapy. St. Edita Stein High School in Kosice, Slovakia, November 2016
  • Huntosova V.: A light helps to heal: Photodynamic Therapy. Krompachy, High School in Krompachy, Slovakia, June 2014
  • Huntosova V.: Photo-biophysics: Two faces of oxygen: when a photo-diagnostics is changed to a photo-treatment. Prirodovedecka cajovna at Faculty of Sciences at UPJS in Kosice, 14. May 2014
  • Actual project:

    principal investigator:

    • Euro-BioImaging & ISIDORe Titile of the Project: Interaction of metal organic framework transport system for antivirotics delivery with cells affected by SARS-CoV-2 (2023)
    • Czech bioimaging Title of the Project: Imaging mitochondrial heterogeneity and lipid composition change in glioblastoma cells from 3D spheroids exposed to newly synthesized sphingolipids (2023)

    co-investigator:

    • VEGA 1/0187/23 Title of the Project: Effect of photobiomodulation in 2D and 3D cell model of Parkinson disease (2023-2025)
    • APVV-21-0333 Title of the Project: Flexible microstructures and microrobots for biomedical ‘lab-onchip’ applications (2022-2026)
    • VVGS PCOV vvgs-2022-2408 Title of the Project: Development and study of functional Heusler nanowires for bio-application (2022-2023)
    • APVV-20-0340 Title of the Project: Development of efficient genetically encoded photosensitizers (2021-2024)

     Past projects:

    principal investigator:

    • VEGA1/0156/18 – Title of the Project: Time-resolved imaging of oxygen consumption in cancer cells during photo-dynamic therapy (2018-2020)

  • Sciex-NMSch N° 10.142 SVK – Title of the Project: Measurement of the tissue oxygenation based on the time-resolved detection of the delayed fluorescence of photosensitizers – scientific exchange program NMS-CH 18 months fellowship at EPFL in Lausanne, Switzerland (2011-2013)

  • VVGS PF 11/2009/F Title of the Project: Monitoring the level of intracellular hypericin aggregation by fluorescence recovery measurements – project for young scientist at UPJS (2009-2010)

  • Bourse de cotutelle de thèse BGF – Fellowship from French Government for double diploma PhD at UPJŠ in Košice and UPMC in Paris, France (2007-2010)

 co-investigator:

  • H2020-MSCA WIDESPREAD-05-2020: CasProt – Fostering high scientific quality in protein research in Eastern Slovakia, WIDESPREAD-05-2020: Twinning (2020-2023)
  • OPII-VA/DP/2020/9.4-01: BioPickmol – Development of nanosensor photonic systems for fast detection of viruses using directed protein evolution methods, ITMS2014+: 313011AUW6 (2021-2023)
  • OPVaI-VA/DP/2018/1.2.1-08: Open scientific community for modern interdisciplinary research in medicine (Acronym: OPENMED), ITMS2014+: 313011V455 supported by the Operational Programme Integrated Infrastructure (2019-2023)
  • COST Action CA17121 – Title: Correlated multimodal imaging in life sciences (COMULIS) (2018-2022) – management committee member for Slovakia
  • VEGA1/0421/18 – Title of the Project: Photobiostimulation as a mitochondrial dysfunction therapy (PHOBIOPIA) (2018-21)
  • APVV-15-0485 – Title of the Project: Towards highly selective cancer treatment: endogenous lipoprotein-DARPin complexes as a new generation of targeted drug delivery vehicles (2016-2020)

  • VEGA1/0156/18 – Title of the Project: Time-resolved imaging of oxygen consumption in cancer cells during photo-dynamic therapy (2018-2020)
  • VEGA 1/0425/15 – Molecular mechanisms of endothelial cell responses to oxidative stress at hypoxia (HYPOXENCELL) (2015-2017)
  • 7RP EÚ „Fostering Excellence in Multiscale Cell Imaging“ (CELIM) (2013-2016)
  • OPVaV – 2009/2.1/02-SORO: CEVA Centres of excellence for atherosclerosis research, ITMS 26220120040 (2010-2013)
  • APVV-LPP 0337-06 Photodynamic therapy of cancer in the light of new molecular knowledge (2006-2010)
  • APVV-0449-07 Targeted photodynamic therapy of cancer: from drug transport through cell signaling pathways up to in vivo model. (2008-2010)

Collaborations:

Peer-review publications (50):

 2023

1) Siposova K., Huntosova V., Sedlakova D., Macajova M., Bilcik B., Nair A., Nair S., Hovhannisyan V., Chen S., Musatov A.: Biocompatible zeolite-dye composites with anti-amyloidogenic properties. International Journal of Biological Macromolecules 251: 126331 (2023) Q1 IF8.025

2) Pevna V., Zauška Ľ., Benziane A., Vámosi G., Girman V., Miklóšová M., Zeleňák V., Huntošová V.*, Almáši M.: Effective transport of aggregated hypericin encapsulated in SBA-15 nanoporous silica particles for photodynamic therapy of cancer cells. Journal of Photochemistry and Photobiology B: Biology 247: 112785 (2023) Q1 IF6.814

3) Pevna V., Zauska L., Almasi M., Hovan A., Bano G., Macajova M., Bilcik B., Zelenak V., Huntosova V.*: Redistribution of hydrophobic hypericin from nanoporous particles of SBA-15 silica in vitro, in cells and in vivo. International Journal of Pharmaceutics 643: 123288 (2023) Q1 IF5.8

4) Hovan A., Pevna V., Huntosova V., Miskovsky P., Bano G.: Singlet oxygen lifetime changes in dying glioblastoma cells. Photochemistry and Photobiology published online (2023) Q2 IF3.3

5) Pevná V., Wagnieres G., Jancura D., Huntosova V.*: Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG Cells. Cells 12(10): 1441 (2023) Q1 IF7.

6) Huntosova V., Andreana M., Unterhuber A.: Editorial: Advanced imaging and its application in biology and medicine. Frontiers in Cell and Developmental Biology 11:1163210 (2023) Q1 IF6.081

 2022

1) Datta S.*, Huntosova V.*, Jutkova A., Seliga R., Kronek J., Tomkova A., Lenkavska L., Macajova M., Bilcik B., Kundekova B., Cavarga I., Pavlova E., Sloufl M., Miskovsky P., Jancura D.: Influence of hydrophobic side-chain length in amphiphilic gradient copoly(2-oxazoline)s on the therapeutics loading, stability, cellular uptake and pharmacokinetics of nano-formulation with curcumin. Pharmaceutics 14, 2576 (2022) Q1 IF6.525

2) Kundekova B., Macajova M., Meta M., Cavarga I., Huntosova V., Datta S., Miskovsky P., Kronek J., Bilcik B.: The Japanese quail chorioallantoic membrane as a model to study an amphiphilic gradient copoly(2-oxazoline)s- based drug delivery system for photodynamic diagnosis and therapy research. Photodiagnosis and Photodynamic Therapy. 40:103046 (2022) Q2 IF3.631

3) Pevna V., Macajova M., Hovan A., Bano G., Meta M., Bilcik B., Palkova J., Huntosova V.*: Spheroidal Model of SKBR3 and U87MG Cancer Cells for Live Imaging of Caspase-3 during Apoptosis Induced by Singlet Oxygen in Photodynamic Therapy. Biomedicines 10:2141 (2022) Q2 IF4.757

4) Pevna V., Horvath D., Wagnieres G., Huntosova V.*: Photobiomodulation and photodynamic therapy-induced switching of autophagy and apoptosis in human dermal fibroblasts. Journal of Photochemistry and Photobiology, B: Biology. 234:112539 (2022) Q1 IF6.814

5) Siposova K.*, Huntosova V.*, Garcarova I., Shlapa Y., Timashkov I., Belous A., Musatov A.: Dual-Functional Antioxidant and Antiamyloid Cerium Oxide Nanoparticles Fabricated by Controlled Synthesis in Water-Alcohol Solutions. Biomedicines, 10(5):942 (2022) Q2 IF4.757

6) Máčajová M., Huntošová V.*, Meta M., Kundeková B., Bilčík B*.: Quail Chorioallantoic Membrane – A Tool for Photodynamic Diagnosis and Therapy. Journal of Visualized Experiments, e63422 (2022) Q2 IF1.4

 2021

1) Pevna V., Wagnieres G., Huntosova V.*: Autophagy and Apoptosis Induced in U87 MG Glioblastoma Cells by Hypericin-Mediated Photodynamic Therapy Can Be Photobiomodulated with 808 nm Light. biomedicines, 9:1703 (2021) Q1 IF6.081

2) Huntosova V.*, Datta S., Lenkavska L., Macajova M., Bilcik B., Kundekova B., Cavarga I., Kronek J., Jutkova A., Miskovsky P., Jancura D.: Alkyl Chain Length in Poly(2-oxazoline)-Based Amphiphilic Gradient Copolymers Regulates the Delivery of Hydrophobic Molecules: A Case of the Biodistribution and the Photodynamic Activity of the Photosensitizer Hypericin. Biomacromolecules, 22: 4199-4216 (2021) Q1 IF6.092

3) Horvath D.*, Tomkova S., Huntosova, V.*: Selection of Unique Molecules for Cancer Treatment by Distance-Based Method: Hypericin Effect on Respiratory Chain. Biophysica, 1:222-237 (2021) Q- IF-

4) Beňová E., Horenbecq V., Zeleňák V., Huntošová V., Almáši M., Máčajová M., Bergé-Lefranc D.: pH-responsive mesoporous silica drug delivery system, its biocompatibility and co-adsorption/co-release of 5-Fluorouracil and Naproxen. Applied Surface Science, 561:150011 (2021) Q1 IF6.182

5) Moskvin M., Huntosova V., Herynek V., Matous P., Michalcova A., Lobaz V., Zasonska B., Slouf M., Seliga R., Horak D.: In vitro cellular activity of maghemite/cerium oxide magnetic nanoparticles with antioxidant properties. Colloids and Surface B: Biointerfaces, 204:111824 (2021) Q1 IF4.389

6) Zeleňáková A., Szűcsová J., Nagy Ľ, Girman V., Zeleňák V., Huntošová V.: Magnetic characterization and moderate cytotoxicity of magnetic mesoporous silica nanocomposite for drug delivery of naproxen. nanomaterials, 11:901 (2021) Q1 IF5.076

7) Rendošová M., Gyepes R., Maruščáková IC., Mudroňová D., Sabolová D., Kello M., Vilková M., Almáši M., Huntošová V., Zemek O., Vargová Z.: An in vitro selective inhibitory effect of silver(I) aminoacidates against bacteria and intestinal cell lines and elucidation of the mechanism of action by means of DNA binding properties, DNA cleavage and cell cycle arrest. Dalton Transactions, 50:936-953 (2021) Q1 IF 4.174

8) Huntosova V.*, Horvath D., Seliga R., Wagnieres G.: Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro. Molecules, 26:485 (2021) Q1 IF 3.267

2020

1) Dzupponova V., Huntosova V., Zoldak G.: A kinetic coupling between protein unfolding and aggregation controls time-dependent solubility of the human myeloma antibody light chain. Protein Science, 29:2408-2421 (2020) Q2 IF 3.876

2) Lenkavska L., Tomkova S., Horvath D., Huntosova V.*Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis and Photodynamic Therapy, 31:101813 (2020) Q2 IF 2.589

3) Almasi M., Benova E., Zelenak V., Madaj B., Huntosova V., Brus J., Urbanova M., Bednarcik J., Hornebecq V.: Cytotoxicity study and influence of SBA-15 surface polarity and pH on adsorption and release properties of anticancer agent pemetrexed. Materials Science and Engineering:C, 109: 110552 (2020) (Cit. 1) Q1 IF 4.959

4) Benova E., Berge-Lefranc D., Zelenak V., Almasi M., Huntosova V., Hornebecq V.: Adsorption properties, the pH-sensitive release of 5-fluorouracil and cytotoxicity studies of mesoporous silica drug delivery matrix. Applied Surface Science; 504:144028 (2020) (Cit. 5) Q1 IF 5.155

 2019

1) Stroffekova K., Tomkova S., Huntosova V., Kozar T.: Importance of Hypericin-Bcl2 interactions for biological effects at subcellula levels. Photodiagnosis and Photodynamic Therapy; 28:38-52 (2019) (Cit. 1) Q2 IF 2.895

2) Siposova K., Huntosova V.*, Shlapa Y., Lenkavska L., Macajova M., Belous A., Musatov A.: Advances in the study of cerium oxide nanoparticles: new insights into antiamyloidogenic activity. ACS Applied Bio Materials; 2:1884-1806 (2019) (Cit. 4) Q- IF-

3) Siposova K., Kozar T., Huntosova V., Tomkova S., Musatov A.: Inhibition of amyloid fibril formation and disassembly of pre-formed fibrils by natural polyphenol rottlerinBiochimica et Biophysica Acta-Proteins and Proteomics; 25:259-274 (2019) (Cit. 8) Q1 IF 2.609

4) Lenkavska L., Blascakova L., Jurasekova Z., Macajova M., Bilcik B., Cavarga I., Miskovsky P., Huntosova V.*Benefits of hypericin transport and delivery by low- and high-density lipoproteins to cancer cells: From in vitro to ex ovoPhotodiagnosis and Photodynamic Therapy; 25: 214-224 (2019) (Cit. 3) Q2 IF 2.895

 2018

1) Blascakova L., Horvath D., Belej D., Wagnieres G., Miskovsky P., Jancura D., Huntosova V.*Hypericin can cross barriers in the chicken’s chorioallantoic membrane model when delivered in low-density lipoproteinsPhotodiagnosis and Photodynamic Therapy; 23:306-313 (2018) (Cit. 1) Q2 IF 2.895

2) Datta S., Jutkova A., Sramkova P., Lenkavska L., Huntosova V., Chorvat D., Miskovsky P., Jancura D., Kronek J.: Unravelling the excellent chemical stability and bioavailability of solvent responsive curcumin-loaded 2-ethyl-2-oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline copolymer nanoparticles for drug deliveryBiomacromolecules; 19:2459-2471 (2018) (Cit. 11) Q1 IF 5.738

3) Tomkova S., Misuth M., Lenkavska L., Miskovsky P., Huntosova V.*In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cellsBiochimica et Biophysica Acta-Molecular Cell Research; 1865:616-628 (2018) (Cit. 5) Q1 IF 4.521

 2017

1) Belej D., Jurasekova Z., Nemergut M., Wagnieres G., Jancura D., Huntosova V.*Negligible interaction of [Ru(Phen)3]2+ with human serum albumin makes it promising for a reliable in vivo assessment of the tissue oxygenationJournal of Inorganic Biochemistry; 174:37-44 (2017) (Cit. 2) Q2 IF 2.248

2) Huntosova V.*, Gerelli E., Horvath D., Wagnieres G.: Measurement of pO2 by luminescence lifetime spectroscopy: A comparative study of the phototoxicity and sensitivity of [Ru(Phen)3]2+ and PdTCPP in vivoJ. Biophotonics; 10(5):708-717 (2017) (Cit. 4) Q1 IF 3.763

3) Misuth M., Horvath D., Miskovsky P., Huntosova V.*Synergism between PKCδ regulators hypericin and rottlerin enhances apoptosis in U87 MG glioma cells after light stimulationPhotodiagnosis and Photodynamic Therapy; 18:267-274 (2017). (Cit. 3) Q2 IF 2.895

4) Misuth M., Joniova J., Belej D., Hrivnak S., Horvath D., Huntosova V.*Estimation of PKCδ autophosphorylation in U87 MG glioma cells: combination of experimental, conceptual and numerical approachesJ. Biophotonics; 10(3):423-432 (2017). (Cit. 1) Q1 IF 3.763

5) Joniova J., Rebic M., Strejckova A., Huntosova V., Stanicova J., Jancura D., Miskovsky P., Bano G.: Formation of large hypericin aggregates in giant unilamellar vesicles-experiments and modelingBiophysical journal; 112(5):966-975 (2017) (Cit. 4) Q1 IF 3.665

6) Misuth M., Joniova J., Horvath D., Dzurova L., Nichtova Z., Novotova M., Miskovsky P., Stroffekova K., Huntosova V.*The flashlights on a distinct role of protein kinase C δ: Phosphorylation of regulatory and catalytic domain upon oxidative stress in glioma cellsCellular Signalling; 34:11-22 (2017) (Cit. 4) Q2 IF 3.968

7) Huntosova V., Novotova M., Nichtova Z., Balogova L., Maslanakova M., Petrovajova D., Stroffekova K.: Assessing light-independent effects of hypericin on cell viability, ultrastructure and metabolism in human glioma and endothelial cellsToxicology in Vitro; 40:184-195 (2017) (Cit. 10) Q1 IF 2.903

 2016

1) Huntosova V., Stroffekova K.: Hypericin in the Dark: Foe or Ally in Photodynamic Therapy?Cancers; 8(10),93 (2016) (Cit. 6) Q1 IF 6.126

2) Huntosova V.*, Gerelli E., Zellweger M., Wagnieres G.: Effect of PpIX photoproducts formation on pO2 measurement by time-resolved delayed fluorescence spectroscopy of PpIX in solution and in vivoJ. Photoch Photobio B;164:49-56 (2016) (Cit. 4) Q1 IF 4.383

3) Verebova V., Belej D., Joniova J., Jurasekova Z., Miskovsky P., Kozar T., Horvath D., Stanicova J., Huntosova V.*Deeper insights into the drug defense of glioma cells against hydrophobic moleculesInt. J. Pharmaceutics; 503(1-2):56-67 (2016) (Cit. 1) Q1 IF 4.845

 2014

1) Huntosova V.*, Stroffekova K., Wagnieres G., Novotova M., Nichtova Z., Miskovsky P.: Endosomes: guardians against [Ru(Phen)3]2+ photo-action in endothelial cells during in vivo pO2 detection?Metallomics; 6(12): 2279-2289 (2014) Q1 IF 3.796

2) Varchola J., Huntosova V., Jancura D., Wagnieres G., Miskovsky P., Bano G.: Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitationPhotochem. Photobiol. Sci.; 13(12): 1781-1787 (2014) (Cit. 2) Q2 IF 2.902

3) Huntosova V., Gay S., Nowak-Sliwinska P., Rajendran SK., Zellweger M., van den Bergh H., Wagnieres G.; In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrateJ. Biomed. Opt.; 19(7): 077004 (2014) (Cit. 6) Q1 IF 2.785

4) Dzurova L., Petrovajova D., Nadova Z., Huntosova V., Miskovsky P., Stroffekova K.: The role of anti-apoptotic protein kinase C alpha in response to hypericin photodynamic therapy in U-87 MG cellsPhotodiagn Photodyn; 11(2): 213-226 (2014) (Cit. 4) Q2 IF 2.895

 2012

1) Huntosova V, Buzova D, Petrovajova D, Kasak P, Nadova Z, Jancura D, Sureau F, Miskovsky P.: Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cellsInt. J. Pharmaceutics; 436 (1-2): 463-71 (2012) (Cit. 39) Q1 IF 4.845

2) Huntosova V, Nadova Z, Dzurova L, Jakusova V, Sureau F, Miskovsky P, : Cell death response of U87 glioma cells on hypericin photoactivation is mediated by dynamics of hypericin subcellular distribution and its aggregation in cellular organellesPhotochem. Photobiol. Sci.; 11 (9): 1428-36 (2012) (Cit. 19) Q2 IF 2.902

 2010

1) Huntosova V, Alvarez L, Bryndzova L, Nadova Z, Jancura D, Buriankova L, Bonneau S, Brault D, Miskovsky P, Sureau F, : Interaction dynamics of Hyp with low-density lipoproteins and U87-MG cellsInt. J. Pharmaceutics ; 389: 32-40 (2010) (Cit. 12) Q1 IF 4.845

2) Balint S, Rao S, Sanchez M, Huntosova V, Miskovsky P, Petrov D, : Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probesJ. Biomedical Optics; 15 (2): 027005 (2010) (Cit. 13) Q1 IF 2.785

2008

1) Kascakova S, Nadova Z, Mateasik A, Mikes J, Huntosova V, Refregiers M, Sureau F, Maurizot JC, Miskovsky P, Jancura D, : High- level of low-density lipoprotein receptors enhance Hyp uptake by U-87 MG cells in the presence of LDLPhotochem Photobiol. ; 84 (1): 120-127 (2008) (Cit. 17) Q1 IF 2.214

 

Book Chapter (1):

1) Huntosova V.* and Wagnieres, G.: Chapter 15: pO2 measurements in biological tissues by luminescence lifetime spectroscopy: strategies to exploit or minimize phototoxic effects in tumors. RSC Detection Science, 11:298-318 (2018invited

 

Conference Papers (11):

 2023

1) Zeleňáková A., Szucsova J., Huntošová V., Hrubovčák P., Zeleňák V.: Magnetic properties and cytotoxicity study of iron oxide nanoparticles with gold layer. AIP Conferece Proceedings; 040029 (2023)

2) Olejárová S., Huntošová V.: Účinok fotobiomodulačného žiarenia po hypericínom indukovanej fotodynamickej terapii v nádorových a nenádorových bunkách in vitro. Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; p.433-438 ISBN 9788022356084 (2023)

3) Pevná V., Zauška Ľ., Almáši M., Zeleňák V., Miškovský P., Huntošová V.: Interakcia fluorescenčných molekúl aktívnych vo fotodynamickej terapii s mezopórovitou silikou a bunkami. Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; p. 473-478 ISBN 9788022356084 (2023)

 2022

1) Pevná V., Miškovský P., Huntošová V.: Identifikácia fosforylácie Proteín Kinázy Cδ v izolovaných frakciách buniek vystavených fotobiomodulácii pri 808 nm. Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; p. 503-508, ISBN 9788022353854 (2022)

 2021

1) Jaďuďová D., Šipošová K., Huntošová V.: Vplyv nanocérií na detekciu hypericínu a fotodynamickú terapiu nádorových buniek. Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; p.174-179. ISBN 9788022351324 (2021)

 2020

1) Kundekova B., Meta M., Huntosova V., Cavarga I., Kronek J., Bilcik B., Macajova M.: Porovnanie farmakokinetiky fotoaktivnych latok hypericinu a kurkuminu v kombinacii s polymernymi nanocasticami na modeli prepelicej CAM; Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; 303-308, ISBN 978-80-223-5032-7 (2020)

 2019

1) Huntošova V.*, Seliga R., Horváth D.: Time-resolved measurements of tissue oxygenation and oxidative stress level in cancer cells. In Genetic toxicology and cancer prevention :  Bilateral  Czech  and  Slovak  Workshop – Bratislava  :  Cancer  Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, p. 62-67. ISBN 978-80-972247-4-5 (2019)

2) Kundekova B., Meta M., Huntosova V., Bilcik B., Macajova M.: Vplyv nadorovych buniek a hypericinu na expresiu genov dolezitych pre angiogenezu na modeli prepelicej chorioalantoickej membrany; Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; 332-337, ISBN 978-80-223-4711-2 (2019)

 2017

1) Gerelli E., Huntosova V., Horvath D., Wagnieres G.: In vivo measurement of the tissue oxygenation by time-resolved luminescence spectroscopy of protoporphytin IX: Strategies to minimize artefacts associated with photoproducts; Proc. SPIE 10060:1006016, Optical Biopsy XV: Toward real-time spectroscopic imaging and diagnosis. (2017)

 2016

1) Jurasekova Z., Huntosova V., Belej D., Miskovsky P., Jancura S.: Human serum albumin-[Ru(Phen)3]2+complex formation studied by optical spectroscopies; Biophysical Journal-10.1016/j.bpj.2015.11.325, 110:48A-48A (2016) (Cit. 1)

 2012

1) Buzova D, Huntosova V, Kasak P, Petrovajova D, Joniova J, Dzurova L, Nadova Z, Sureau F, Miskovsky P, Jancura D.: Towards increased selectivity of drug delivery to cancer cells: development of a LDL-based nanodelivery system for hydrophobic photosensitizers; Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600U (2012)

  • L’ORÉAL-UNESCO for women in Science an honorable mention for project entitled: Targeted anti-cancer drug delivery to breast cancer (2018)
  • Slovak Biophysical Society’s young scientist award (under 35 years old) for the scientific work in the field of Biophysics in years 2014, 2015. (April 2016)
  • IUPAC (International Union of Pure and Applied Chemistry) award for the best poster presentation at 16th International Congress on Photobiology, (September 2014), Cordoba, Argentina. Poster presentation: Effect of ROS created during pO2 detection by [Ru(Phen)3]2+ luminescence lifetime measurements
  • CSEM award for the best oral or poster presentation by young researcher at the yearly meeting of the Biomedical Photonics Network: Progress in Biomedical Photonics, (November 2011), Neuchatel, Switzerland. Poster presentation: Assessment of the tissue oxygenation in vivo by measuring the luminescence lifetime of Ru(Phen)32+ as poorly photosensitizing probe
  • The Slovak-Czech woman foundation for young women in science. The fellowship of Ľudmila Čuchranova for attending the 13th Congress of European Society for Photobiology in conjunction with the 2nd conference of the European Platform for Photodynamic Medicine, 5-10 September 2009, Wroclaw, Poland.
  • The fellowship of Socrates/Erasmus program. The fellowship for 3 months stay at the University of Pierre and Marie Curie in Paris, France. April-June 2008
  • The European Society for Photobiology (ESP). The fellowship for attending the 34th Meeting of the American Society for Photobiology (ASP) in Burlingame, California, USA, 20-25 June 2008
  • The ESP fellowship for attending the 12th Congress of ESP in Bath, UK, 1-6 September 2007
  • Honourable mention of the president of Faculty of Science, Pavol Jozef Safarik University in Košice for the scientific work (2006).
  • Slovak literary found award for the excellent student scientific work entitled: Spectroscopic study of influence HSA and LDL to accumulation and distribution hypericin in cell U-87 MG (2006)
  • Diploma of the 1st place in the Biophysical and Biomedical section at the National Student Scientific Conference in Bratislava (2006).
  • Diploma of the 2nd place in Biochemical section at Student Scientific Conference in Košice (2006).