RNDr. Veronika Huntošová, PhD.

Current positions: 

  • researcher – head of Confocal microscopy and Time resolved microscopy laboratories


2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics
2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics
2008 Safarik University Kosice – rigorous exam RNDr. in Physic

2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et  Marie Curie ED 387

Research interests:

  • Monitoring of cell/tissue oxygenation and oxidative stress by steady state and time-resolved microscopy;
  • Monitoring of protein kinase C signaling related to cell survival and apoptosis;
  • Development of nanoscale transport systems for cancer targeting;
  • Appication of avian chorioallantoic membrane model for photodiagnosis, photodynamic therapy, and tumor targeting;


2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics
2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics
2008 Safarik University Kosice – rigorous exam RNDr. in Physics

2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et Marie Curie ED 387

Professional positions:

2010 – 2011 Research fellow, Department of Biophysics, Safarik University Kosice
2011 – 2013 Post-Doc fellow, Ecole Polytechnique Federale de Lausanne (Switzerland)
2013 – Research fellow, Center for Interdisciplinary Biosciences, Technology and innovation park, Safarik University Kosice

Research training:

2005 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2006 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2008 Pierre et Marie Curie University in Paris 6 (France) (3 months)
2010 EBSA Biophysics Course in Bordeaux-Arcachon, France (1 week)
2012 ESP Photobiology School in Brixen/Bressanone, Italy (1 week)
2013-2016 Swiss federal institute of technology (EPFL) in Lausanne (Switzerland) (4 x 1 month)
2013-2016 Pierre et Marie Curie University in Paris 6 (France) (3x 1 month)


UV-VIS absorption spectroscopy, fluorescence spectroscopy, time-resolved spectroscopy/microscopy, fluorescence and fluorescence confocal-microscopy, stopped-flow spectroscopy, flow-cytometry analysis of cellular processes, cells viability tests, isolation of cellular structures, immunifluorescence, western blotting, cultivation of cells cultures, incubation and preparation of quail, turkey and chick’s chorioallantoic membranes (CAM) in vivo, tests of phototoxicity in vivo in the CAM model.


Number of articles in journals registered in WoS and SCOPUS: 35
Number of SCI citations (self-citations excluded): 241
H-index: 10

Membership in scientific societies:

2017-present Czechoslovak Microscopy society and Microscopy society
2015-present Biophysical Society-USA and Europe
2015-present Slovak Biophysical Society (SKBS), from 2017-2020 a member of a SKBS board, 2020-secretary of SKBS 2012-2013 International society on oxygen transport to tissue (ISOTT)

2006-2013 European Society for Photobiology (ESP)



ÚFV/FChFB/14 – Fotochémia a fotobiológia

ÚFV/CB/14 – Bunková biológia

ÚFV/EMBF3/18 – Experimentálne metódy biofyziky III

ÚFV/PEMBF3/18 – Praktikum k experimentálnym metódam biofyziky III


Thesis supervisions:


  • Tomkova: Role of inter-organelle communication in cell function. Co-supervisor (in progress)
  • Lenkavska: Develompment of transport systems for cancer targeting. Supervisor (in progress)


  • Blascakova: Development of drug nanodelivery vehicles based on lipids, lipoproteins and saponins. Co-supervisor (completed)


  • Verebova: Investigation of interactions between biological macromolecules and photoactive molecules. Co-supervisor (completed)

Master’s thesis supervisions:


  • Jadudova: Investigation of nanosensors for monitoring of tissues and cells oxygenation and the level of oxidative stress in cancer cells (in progress)


  • Ambrozova: The study of Darpins biodistribution and their influence on cancer cells viability in vitro and ex ovo: the quail chorioallantoic membrane model (completed)


  • Jenikova: Influence of environment and photoproducts on PpIX luminescence detection (completed)

Bachelor’s thesis supervisions:


  • Lukacova: The study of the luminescence oxygen sensitive probes (in progress)


  • Jadudova: The study of CeO2 nanoparticles uptake and cytotoxic effect on cancer cells (completed)
  • Abdessamad: Monitoring of oxidative damage in cells during photodynamic therapy (completed)

Secondary school student scientific work supervisions:


  • Burdova: Využitie kombinácie acidifikácie extracelulárneho pH nádorových buniek a účinkov rottlerinu ako jedného zo spôsobov protinádorovej terapie. Achievement: AMAVET Diploma, and certificate to participate at EU contest for young scientist EUCYS in Salamanca, Spain 2021)
  • Burdova: Využitie kombinácie acidifikácie extracelulárneho pH nádorových buniek a účinkov rottlerinu ako jedného zo spôsobov protinádorovej terapie. 1st place at the regional secondary school student’s competition


  • Nagyova, K. Mitrova a G. Mitrova: Interakcia hypericínu s kvasinkami.


  • Motesicka: Influence of PKCδ regulators on photodynamic therapy efficacy. EU contest for young scientist EUCYS in Dublin, Ireland 2018. Achievement: EUCYS 2018 prize winner entitled her to attend a conference at EMBL, Heidelberg); interview in press SK
  • Motesicka: Prírodné prvky ako liek alebo prevencia proti rakovine. 1st place at the regional secondary school student’s competition
  • Mitrova a B. Nagyova: Využitie ľubovníka bodkovaného vo fotodynamickej terapii. 1st place at the regional secondary school student’s competition.
  • Korossyova a M. Adamjak: Charakterizácia nádorových sféroidov


Invited lectures:

  • Huntosova V.: Detection of singlet oxygen using time-resolved techniques. LLE TReT workshop on Time-resolved techniques, 20-22th June 2018, BioCeV, Vestec, Czech Republic
  • Huntosova V.: Mitochondria and lysosomes movement during photodynamic action in living cells. Andor Academy, 7-9th November 2017, Center of Biosciences SAS in Bratislava, Slovakia
  • Huntosova V.: Reorganization and dynamics of subcellular organelles during anticancer treatment. Nanoscopy studies vol. 1 – Teoretical and practical sessions with Leica STED 3X, 19-21st September 2017, Center of Biosciences SAS in Bratislava, Slovakia
  • Huntosova V., Sokolova S., Lenkavska L., Miskovsky P.: Time-resolved visualization of oxidative stress during apoptosis in cancer cells by fluorescent probes. 9-10th May 2017, Bratislava, Slovakia
  • Huntosova V.: Drug delivery systems and oxygen detection in photodynamic therapy and photodyagnostics. 7th Slovak Biophysical Symposium, Novy Smokovec, Slovakia, 6-8. April 2016
  • Huntosova V.: Photons in a way to study biostructures, ALUMNI SPACE, P.J. Safarik University in Kosice, Slovakia, 6. February 2015

Popularization lectures:

  • Huntosova V.: A light helps to heal: Photodynamic Therapy. St. Edita Stein High School in Kosice, Slovakia, November 2016
  • Huntosova V.: A light helps to heal: Photodynamic Therapy. Krompachy, High School in Krompachy, Slovakia, June 2014
  • Huntosova V.: Photo-biophysics: Two faces of oxygen: when a photo-diagnostics is changed to a photo-treatment. Prirodovedecka cajovna at Faculty of Sciences at UPJS in Kosice, 14. May 2014

Actual project:

principal investigator:


Past projects:

principal investigator:

  • VEGA1/0156/18 – Title of the Project: Time-resolved imaging of oxygen consumption in cancer cells during photo-dynamic therapy (2018-2020)

  • Sciex-NMSch N° 10.142 SVK – Title of the Project: Measurement of the tissue oxygenation based on the time-resolved detection of the delayed fluorescence of photosensitizers – scientific exchange program NMS-CH 18 months fellowship at EPFL in Lausanne, Switzerland (2011-2013)

  • VVGS PF 11/2009/F Title of the Project: Monitoring the level of intracellular hypericin aggregation by fluorescence recovery measurements – project for young scientist at UPJS (2009-2010)


  • APVV-15-0485 – Title of the Project: Towards highly selective cancer treatment: endogenous lipoprotein-DARPin complexes as a new generation of targeted drug delivery vehicles (2016-2020)

  • 7RP EÚ „Fostering Excellence in Multiscale Cell Imaging“ (CELIM) (2013-2016)


Peer-review publications (32):


1) Rendošová M., Gyepes R., Maruščáková IC., Mudroňová D., Sabolová D., Kello M., Vilková M., Almáši M., Huntošová V., Zemek O., Vargová Z.: An in vitro selective inhibitory effect of silver(I) aminoacidates against bacteria and intestinal cell lines and elucidation of the mechanism of action by means of DNA binding properties, DNA cleavage and cell cycle arrest. Dalton Transactions, 50:936-953 (2021) Q1 IF 4.174

2) Huntosova V.*, Horvath D., Seliga R., Wagnieres G.: Influence of Oxidative Stress on Time-Resolved Oxygen Detection by [Ru(Phen)3]2+ In Vivo and In Vitro. Molecules, 26:485 (2021) Q1 IF 3.267


1) Dzupponova V., Huntosova V., Zoldak G.: A kinetic coupling between protein unfolding and aggregation controls time-dependent solubility of the human myeloma antibody light chain. Protein Science, 29:2408-2421 (2020) Q2 IF 3.876

2) Lenkavska L., Tomkova S., Horvath D., Huntosova V.*: Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis and Photodynamic Therapy, 31:101813 (2020) Q2 IF 2.589

3) Almasi M., Benova E., Zelenak V., Madaj B., Huntosova V., Brus J., Urbanova M., Bednarcik J., Hornebecq V.: Cytotoxicity study and influence of SBA-15 surface polarity and pH on adsorption and release properties of anticancer agent pemetrexed. Materials Science and Engineering:C, 109: 110552 (2020) (Cit. 1) Q1 IF 4.959

4) Benova E., Berge-Lefranc D., Zelenak V., Almasi M., Huntosova V., Hornebecq V.: Adsorption properties, the pH-sensitive release of 5-fluorouracil and cytotoxicity studies of mesoporous silica drug delivery matrix. Applied Surface Science; 504:144028 (2020) (Cit. 5) Q1 IF 5.155


1) Stroffekova K., Tomkova S., Huntosova V., Kozar T.: Importance of Hypericin-Bcl2 interactions for biological effects at subcellula levels. Photodiagnosis and Photodynamic Therapy; 28:38-52 (2019) (Cit. 1) Q2 IF 2.895

2) Siposova K., Huntosova V.*, Shlapa Y., Lenkavska L., Macajova M., Belous A., Musatov A.: Advances in the study of cerium oxide nanoparticles: new insights into antiamyloidogenic activity. ACS Applied Bio Materials; 2:1884-1806 (2019) (Cit. 4) Q- IF-

3) Siposova K., Kozar T., Huntosova V., Tomkova S., Musatov A.: Inhibition of amyloid fibril formation and disassembly of pre-formed fibrils by natural polyphenol rottlerin; Biochimica et Biophysica Acta-Proteins and Proteomics; 25:259-274 (2019) (Cit. 8) Q1 IF 2.609

4) Lenkavska L., Blascakova L., Jurasekova Z., Macajova M., Bilcik B., Cavarga I., Miskovsky P., Huntosova V.*: Benefits of hypericin transport and delivery by low- and high-density lipoproteins to cancer cells: From in vitro to ex ovo; Photodiagnosis and Photodynamic Therapy; 25: 214-224 (2019) (Cit. 3) Q2 IF 2.895


1) Blascakova L., Horvath D., Belej D., Wagnieres G., Miskovsky P., Jancura D., Huntosova V.*: Hypericin can cross barriers in the chicken’s chorioallantoic membrane model when delivered in low-density lipoproteins; Photodiagnosis and Photodynamic Therapy; 23:306-313 (2018) (Cit. 1) Q2 IF 2.895

2) Datta S., Jutkova A., Sramkova P., Lenkavska L., Huntosova V., Chorvat D., Miskovsky P., Jancura D., Kronek J.: Unravelling the excellent chemical stability and bioavailability of solvent responsive curcumin-loaded 2-ethyl-2-oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline copolymer nanoparticles for drug delivery; Biomacromolecules; 19:2459-2471 (2018) (Cit. 11) Q1 IF 5.738

3) Tomkova S., Misuth M., Lenkavska L., Miskovsky P., Huntosova V.*: In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cells; Biochimica et Biophysica Acta-Molecular Cell Research; 1865:616-628 (2018) (Cit. 5) Q1 IF 4.521


1) Belej D., Jurasekova Z., Nemergut M., Wagnieres G., Jancura D., Huntosova V.*: Negligible interaction of [Ru(Phen)3]2+ with human serum albumin makes it promising for a reliable in vivo assessment of the tissue oxygenation; Journal of Inorganic Biochemistry; 174:37-44 (2017) (Cit. 2) Q2 IF 2.248

2) Huntosova V.*, Gerelli E., Horvath D., Wagnieres G.: Measurement of pO2 by luminescence lifetime spectroscopy: A comparative study of the phototoxicity and sensitivity of [Ru(Phen)3]2+ and PdTCPP in vivo; J. Biophotonics; 10(5):708-717 (2017) (Cit. 4) Q1 IF 3.763

3) Misuth M., Horvath D., Miskovsky P., Huntosova V.*: Synergism between PKCδ regulators hypericin and rottlerin enhances apoptosis in U87 MG glioma cells after light stimulation; Photodiagnosis and Photodynamic Therapy; 18:267-274 (2017). (Cit. 3) Q2 IF 2.895

4) Misuth M., Joniova J., Belej D., Hrivnak S., Horvath D., Huntosova V.*: Estimation of PKCδ autophosphorylation in U87 MG glioma cells: combination of experimental, conceptual and numerical approaches; J. Biophotonics; 10(3):423-432 (2017). (Cit. 1) Q1 IF 3.763

5) Joniova J., Rebic M., Strejckova A., Huntosova V., Stanicova J., Jancura D., Miskovsky P., Bano G.: Formation of large hypericin aggregates in giant unilamellar vesicles-experiments and modeling; Biophysical journal; 112(5):966-975 (2017) (Cit. 4) Q1 IF 3.665

6) Misuth M., Joniova J., Horvath D., Dzurova L., Nichtova Z., Novotova M., Miskovsky P., Stroffekova K., Huntosova V.*: The flashlights on a distinct role of protein kinase C δ: Phosphorylation of regulatory and catalytic domain upon oxidative stress in glioma cells; Cellular Signalling; 34:11-22 (2017) (Cit. 4) Q2 IF 3.968

7) Huntosova V., Novotova M., Nichtova Z., Balogova L., Maslanakova M., Petrovajova D., Stroffekova K.: Assessing light-independent effects of hypericin on cell viability, ultrastructure and metabolism in human glioma and endothelial cells; Toxicology in Vitro; 40:184-195 (2017) (Cit. 10) Q1 IF 2.903


1) Huntosova V., Stroffekova K.: Hypericin in the Dark: Foe or Ally in Photodynamic Therapy?; Cancers; 8(10),93 (2016) (Cit. 6) Q1 IF 6.126

2) Huntosova V.*, Gerelli E., Zellweger M., Wagnieres G.: Effect of PpIX photoproducts formation on pO2 measurement by time-resolved delayed fluorescence spectroscopy of PpIX in solution and in vivo; J. Photoch Photobio B;164:49-56 (2016) (Cit. 4) Q1 IF 4.383

3) Verebova V., Belej D., Joniova J., Jurasekova Z., Miskovsky P., Kozar T., Horvath D., Stanicova J., Huntosova V.*: Deeper insights into the drug defense of glioma cells against hydrophobic molecules; Int. J. Pharmaceutics; 503(1-2):56-67 (2016) (Cit. 1) Q1 IF 4.845


1) Huntosova V.*, Stroffekova K., Wagnieres G., Novotova M., Nichtova Z., Miskovsky P.: Endosomes: guardians against [Ru(Phen)3]2+ photo-action in endothelial cells during in vivo pO2 detection?; Metallomics; 6(12): 2279-2289 (2014) Q1 IF 3.796

2) Varchola J., Huntosova V., Jancura D., Wagnieres G., Miskovsky P., Bano G.: Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation; Photochem. Photobiol. Sci.; 13(12): 1781-1787 (2014) (Cit. 2) Q2 IF 2.902

3) Huntosova V., Gay S., Nowak-Sliwinska P., Rajendran SK., Zellweger M., van den Bergh H., Wagnieres G.; In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate; J. Biomed. Opt.; 19(7): 077004 (2014) (Cit. 6) Q1 IF 2.785

4) Dzurova L., Petrovajova D., Nadova Z., Huntosova V., Miskovsky P., Stroffekova K.: The role of anti-apoptotic protein kinase C alpha in response to hypericin photodynamic therapy in U-87 MG cells; Photodiagn Photodyn; 11(2): 213-226 (2014) (Cit. 4) Q2 IF 2.895


1) Huntosova V, Buzova D, Petrovajova D, Kasak P, Nadova Z, Jancura D, Sureau F, Miskovsky P.: Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells; Int. J. Pharmaceutics; 436 (1-2): 463-71 (2012) (Cit. 39) Q1 IF 4.845

2) Huntosova V, Nadova Z, Dzurova L, Jakusova V, Sureau F, Miskovsky P, : Cell death response of U87 glioma cells on hypericin photoactivation is mediated by dynamics of hypericin subcellular distribution and its aggregation in cellular organelles; Photochem. Photobiol. Sci.; 11 (9): 1428-36 (2012) (Cit. 19) Q2 IF 2.902


1) Huntosova V, Alvarez L, Bryndzova L, Nadova Z, Jancura D, Buriankova L, Bonneau S, Brault D, Miskovsky P, Sureau F, : Interaction dynamics of Hyp with low-density lipoproteins and U87-MG cells; Int. J. Pharmaceutics ; 389: 32-40 (2010) (Cit. 12) Q1 IF 4.845

2) Balint S, Rao S, Sanchez M, Huntosova V, Miskovsky P, Petrov D, : Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes; J. Biomedical Optics; 15 (2): 027005 (2010) (Cit. 13) Q1 IF 2.785


1) Kascakova S, Nadova Z, Mateasik A, Mikes J, Huntosova V, Refregiers M, Sureau F, Maurizot JC, Miskovsky P, Jancura D, : High- level of low-density lipoprotein receptors enhance Hyp uptake by U-87 MG cells in the presence of LDL; Photochem Photobiol. ; 84 (1): 120-127 (2008) (Cit. 17) Q1 IF 2.214

Book Chapter (1):

1) Huntosova V.* and Wagnieres, G.: Chapter 15: pO2 measurements in biological tissues by luminescence lifetime spectroscopy: strategies to exploit or minimize phototoxic effects in tumors. RSC Detection Science, 11:298-318 (2018) invited

Conference Papers (6):


1) Kundekova B., Meta M., Huntosova V., Cavarga I., Kronek J., Bilcik B., Macajova M.: Porovnanie farmakokinetiky fotoaktivnych latok hypericinu a kurkuminu v kombinacii s polymernymi nanocasticami na modeli prepelicej CAM; Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; 303-308, ISBN 978-80-223-5032-7 (2020)


1) Huntošova V.*, Seliga R., Horváth D.: Time-resolved measurements of tissue oxygenation and oxidative stress level in cancer cells. In Genetic toxicology and cancer prevention :  Bilateral  Czech  and  Slovak  Workshop – Bratislava  :  Cancer  Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, p. 62-67. ISBN 978-80-972247-4-5 (2019)

2) Kundekova B., Meta M., Huntosova V., Bilcik B., Macajova M.: Vplyv nadorovych buniek a hypericinu na expresiu genov dolezitych pre angiogenezu na modeli prepelicej chorioalantoickej membrany; Zbornik recenzovanych prispevkov, Študentska vedecka konferencia PriF UK; 332-337, ISBN 978-80-223-4711-2 (2019)


1) Gerelli E., Huntosova V., Horvath D., Wagnieres G.: In vivo measurement of the tissue oxygenation by time-resolved luminescence spectroscopy of protoporphytin IX: Strategies to minimize artefacts associated with photoproducts; Proc. SPIE 10060:1006016, Optical Biopsy XV: Toward real-time spectroscopic imaging and diagnosis. (2017)


1) Jurasekova Z., Huntosova V., Belej D., Miskovsky P., Jancura S.: Human serum albumin-[Ru(Phen)3]2+complex formation studied by optical spectroscopies; Biophysical Journal-10.1016/j.bpj.2015.11.325, 110:48A-48A (2016) (Cit. 1)


1) Buzova D, Huntosova V, Kasak P, Petrovajova D, Joniova J, Dzurova L, Nadova Z, Sureau F, Miskovsky P, Jancura D.: Towards increased selectivity of drug delivery to cancer cells: development of a LDL-based nanodelivery system for hydrophobic photosensitizers; Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600U (2012)

  • L’ORÉAL-UNESCO for women in Science an honorable mention for project entitled: Targeted anti-cancer drug delivery to breast cancer (2018)
  • Slovak Biophysical Society’s young scientist award (under 35 years old) for the scientific work in the field of Biophysics in years 2014, 2015. (April 2016)
  • IUPAC (International Union of Pure and Applied Chemistry) award for the best poster presentation at 16th International Congress on Photobiology, (September 2014), Cordoba, Argentina. Poster presentation: Effect of ROS created during pO2 detection by [Ru(Phen)3]2+ luminescence lifetime measurements
  • CSEM award for the best oral or poster presentation by young researcher at the yearly meeting of the Biomedical Photonics Network: Progress in Biomedical Photonics, (November 2011), Neuchatel, Switzerland. Poster presentation: Assessment of the tissue oxygenation in vivo by measuring the luminescence lifetime of Ru(Phen)32+ as poorly photosensitizing probe
  • The Slovak-Czech woman foundation for young women in science. The fellowship of Ľudmila Čuchranova for attending the 13th Congress of European Society for Photobiology in conjunction with the 2nd conference of the European Platform for Photodynamic Medicine, 5-10 September 2009, Wroclaw, Poland.
  • The fellowship of Socrates/Erasmus program. The fellowship for 3 months stay at the University of Pierre and Marie Curie in Paris, France. April-June 2008
  • The European Society for Photobiology (ESP). The fellowship for attending the 34th Meeting of the American Society for Photobiology (ASP) in Burlingame, California, USA, 20-25 June 2008
  • The ESP fellowship for attending the 12th Congress of ESP in Bath, UK, 1-6 September 2007
  • Honourable mention of the president of Faculty of Science, Pavol Jozef Safarik University in Košice for the scientific work (2006).
  • Slovak literary found award for the excellent student scientific work entitled: Spectroscopic study of influence HSA and LDL to accumulation and distribution hypericin in cell U-87 MG (2006)
  • Diploma of the 1st place in the Biophysical and Biomedical section at the National Student Scientific Conference in Bratislava (2006).
  • Diploma of the 2nd place in Biochemical section at Student Scientific Conference in Košice (2006).