RNDr. Veronika Huntošová, PhD.

 

Education:

2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics
2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics
2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et  Marie Curie ED 387
2008 Safarik University Kosice – rigorous exam RNDr. in Physics

 

Research interests:

– Time-resolved imaging of oxidative stress in cells
– Monitoring of signaling pathways resulted in apoptosis in cancer cells
– Molecular mechanisms of photodynamic therapy, visualization of cell response, basic dosimetry
– Monitoring of cell/tissue oxygenation by steady state and time-resolved microscopy

 

Education:

2001 – 2004 Safarik University Kosice (Slovakia) – Bachelor of Science in Physics
2004 – 2006 Safarik University Kosice (Slovakia) – Master of Science in Biophysics
2006 – 2010 Safarik University Kosice (Slovakia) and Pierre et Marie Curie University in Paris (France) – double diploma Philosophiae doctor in biophysics and Docteur de l’Universite Pierre et Marie Curie ED 387
2008 Safarik University Kosice – rigorous exam RNDr. in Physics

Professional positions:

2010 – 2011 Research fellow, Department of Biophysics, Safarik University Kosice
2011 – 2013 Post-Doc fellow, Ecole Polytechnique Federale de Lausanne (Switzerland)
2013 – Research fellow, Center for Interdisciplinary Biosciences, Technology and innovation park, Safarik University Kosice

Research training:

2005 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2006 Institute of Physical Biology, Academic and University centre Nove Hrady, University of South Bohemia (Czech Republic) (1 month)
2008 Pierre et Marie Curie University in Paris 6 (France) (3 months)
2010 EBSA Biophysics Course in Bordeaux-Arcachon, France (1 week)
2012 ESP Photobiology School in Brixen/Bressanone, Italy (1 week)
2013-2016 Swiss federal institute of technology (EPFL) in Lausanne (Switzerland) (4 x 1 month)
2013-2016 Pierre et Marie Curie University in Paris 6 (France) (3x 1 month)

Methodology:

UV-VIS absorption spectroscopy, fluorescence spectroscopy, time-resolved spectroscopy/microscopy, fluorescence and fluorescence confocal-microscopy, stopped-flow spectroscopy, flow-cytometry analysis of cellular processes, cells viability tests, isolation of cellular structures, cultivation of cells cultures, incubation and preparation of chick’s chorioallantoic membranes (CAM) in vivo, tests of phototoxicity in vivo in the CAM model.

PhD supervision:

Supervisor of 1 PhD student and co-supervisor of 4 PhD graduates.

Publications:

Number of articles in journals registered in WoS and SCOPUS: 24
Number of SCI citations (self-citations excluded): 99
H-index: 6

Membership in scientific societies:

2017-present Czechoslovak Microscopy society and Microscopy society
2015-present Biophysical Society-USA and Europe
2015-present Slovak Biophysical Society (SKBS), from 2017 a member of a SKBS board
2006-2013 European Society for Photobiology (ESP)
2012-2013 International society on oxygen transport to tissue (ISOTT)

Actual project:

principal investigator:
VEGA1/0156/18 – Title of the Project: Time-resolved imaging of oxygen consumption in cancer cells during photo-dynamic therapy (2018-2020)
co-investigator:
COST Action CA17121 – Title: Correlated multimodal imaging in life sciences (COMULIS) Management Committee (MC) member for Slovakia (2018-2022)
VEGA1/0421/18 – Title of the Project: Photobiostimulation as a mitochondrial dysfunction therapy (PHOBIOPIA) (2018-21)
APVV-15-0485 – Title of the Project: Towards highly selective cancer treatment: endogenous lipoprotein-DARPin complexes as a new generation of targeted drug delivery vehicles (2016-2020)

Past projects:

principal investigator:
VVGS PF 11/2009/F Title of the Project: Monitoring the level of intracellular hypericin aggregation by fluorescence recovery measurements – project for young scientist at UPJS (2009)
Sciex-NMSch N° 10.142 SVK – Title of the Project: Measurement of the tissue oxygenation based on the time-resolved detection of the delayed fluorescence of photosensitizers – scientific exchange program NMS-CH 18 months fellowship at EPFL in Lausanne, Switzerland (2011-2013)
co-investigator:
1 international projects (7FP EU CELIM 2013-2016) and 5 projects at the national level (APVV, VEGA).

Number of articles in journals registered in WoS and SCOPUS: 24
Number of SCI citations (self-citations excluded): 99
H-index: 6

Book Chapter:

1. Huntosova V.* and Wagnieres, G.: Chapter 15: pO2 measurements in biological tissues by luminescence lifetime spectroscopy: strategies to exploit or minimize phototoxic effects in tumors. RSC Detection Science, 11:298-318 (2018)

Peer-reviewed publications:

1) Siposova K., Kozar T., Huntosova V., Tomkova S., Musatov A.: Inhibition of amyloid fibril formation and disassembly of pre-formed fibrils by natural polyphenol rottlerin; Biochimica et Biophysica Acta-Proteins and Proteomics; https://doi.org/10.1016/j.bbapap.2018.10.002 (2018)

2) Blascakova L., Horvath D., Belej D., Wagnieres G., Miskovsky P., Jancura D., Huntosova V.*: Hypericin can cross barriers in the chicken’s chorioallantoic membrane model when delivered in low-density lipoproteins; Photodiagnosis and Photodynamic Therapy; 23:306-313 (2018)

3) Datta S., Jutkova A., Sramkova P., Lenkavska L., Huntosova V., Chorvat D., Miskovsky P., Jancura D., Kronek J.: Unravelling the excellent chemical stability and bioavailability of solvent responsive curcumin-loaded 2-ethyl-2-oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline copolymer nanoparticles for drug delivery; Biomacromolecules; 19:2459-2471 (2018)

4) Tomkova S., Misuth M., Lenkavska L., Miskovsky P., Huntosova V.*: In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cells; Biochimica et Biophysica Acta-Molecular Cell Research; 1865:616-628 (2018)

5) Belej D., Jurasekova Z., Nemergut M., Wagnieres G., Jancura D., Huntosova V.*: Negligible interaction of [Ru(Phen)3]2+ with human serum albumin makes it promising for a reliable in vivo assessment of the tissue oxygenation; Journal of Inorganic Biochemistry; 174:37-44 (2017)

6) Huntosova V.*, Gerelli E., Horvath D., Wagnieres G.: Measurement of pO2 by luminescence lifetime spectroscopy: A comparative study of the phototoxicity and sensitivity of [Ru(Phen)3]2+ and PdTCPP in vivo; J. Biophotonics; 10(5):708-717 (2017)
Cit. 1) Papkovsky D.B., Dmitriev, R.I.: Imaging of oxygen and hypoxia in cell and tissue samples; Cellular and Molecular Life Sciences; 75(16):2963-2980 (2018)

7) Misuth M., Horvath D., Miskovsky P., Huntosova V.*: Synergism between PKCδ regulators hypericin and rottlerin enhances apoptosis in U87 MG glioma cells after light stimulation; Photodiagnosis and Photodynamic Therapy; 18:267-274 (2017).

8) Misuth M., Joniova J., Belej D., Hrivnak S., Horvath D., Huntosova V.*: Estimation of PKCδ autophosphorylation in U87 MG glioma cells: combination of experimental, conceptual and numerical approaches; J. Biophotonics; 10(3):423-432 (2017).

9) Joniova J., Rebic M., Strejckova A., Huntosova V., Stanicova J., Jancura D., Miskovsky P., Bano G.: Formation of large hypericin aggregates in giant unilamellar vesicles-experiments and modeling; Biophysical journal; 112(5):966-975 (2017)

10) Misuth M., Joniova J., Horvath D., Dzurova L., Nichtova Z., Novotova M., Miskovsky P., Stroffekova K., Huntosova V.*: The flashlights on a distinct role of protein kinase C δ: Phosphorylation of regulatory and catalytic domain upon oxidative stress in glioma cells; Cellular Signalling; 34:11-22 (2017)

11) Huntosova V., Novotova M., Nichtova Z., Balogova L., Maslanakova M., Petrovajova D., Stroffekova K.: Assessing light-independent effects of hypericin on cell viability, ultrastructure and metabolism in human glioma and endothelial cells; Toxicology in Vitro; 40:184-195 (2017)

12) Huntosova V., Stroffekova K.: Hypericin in the Dark: Foe or Ally in Photodynamic Therapy?; Cancers; 8(10),93 (2016)

13) Huntosova V.*, Gerelli E., Zellweger M., Wagnieres G.: Effect of PpIX photoproducts formation on pO2 measurement by time-resolved delayed fluorescence spectroscopy of PpIX in solution and in vivo; J. Photoch Photobio B;164:49-56 (2016)

14) Verebova V., Belej D., Joniova J., Jurasekova Z., Miskovsky P., Kozar T., Horvath D., Stanicova J., Huntosova V.*: Deeper insights into the drug defense of glioma cells against hydrophobic molecules; Int. J. Pharmaceutics; 503(1-2):56-67 (2016)

15) Huntosova V.*, Stroffekova K., Wagnieres G., Novotova M., Nichtova Z., Miskovsky P.: Endosomes: guardians against [Ru(Phen)3]2+ photo-action in endothelial cells during in vivo pO2 detection?; Metallomics; 6(12): 2279-2289 (2014)

16) Varchola J., Huntosova V., Jancura D., Wagnieres G., Miskovsky P., Bano G.: Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation; Photochem. Photobiol. Sci.; 13(12): 1781-1787 (2014)

17) Huntosova V., Gay S., Nowak-Sliwinska P., Rajendran SK., Zellweger M., van den Bergh H., Wagnieres G.; In vivo measurement of tissue oxygenation by time-resolved luminescence spectroscopy: advantageous properties of dichlorotris(1, 10-phenanthroline)-ruthenium(II) hydrate; J. Biomed. Opt.; 19(7): 077004 (2014)

18) Dzurova L., Petrovajova D., Nadova Z., Huntosova V., Miskovsky P., Stroffekova K.: The role of anti-apoptotic protein kinase C alpha in response to hypericin photodynamic therapy in U-87 MG cells; Photodiagn Photodyn; 11(2): 213-226 (2014)

19) Huntosova V, Buzova D, Petrovajova D, Kasak P, Nadova Z, Jancura D, Sureau F, Miskovsky P.: Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells; Int. J. Pharmaceutics ; 436 (1-2): 463-71 (2012)

20) Huntosova V, Nadova Z, Dzurova L, Jakusova V, Sureau F, Miskovsky P, : Cell death response of U87 glioma cells on hypericin photoactivation is mediated by dynamics of hypericin subcellular distribution and its aggregation in cellular organelles; Photochem. Photobiol. Sci.; 11 (9): 1428-36 (2012)

21) Huntosova V, Alvarez L, Bryndzova L, Nadova Z, Jancura D, Buriankova L, Bonneau S, Brault D, Miskovsky P, Sureau F, : Interaction dynamics of Hyp with low-density lipoproteins and U87-MG cells; Int. J. Pharmaceutics ; 389: 32-40 (2010)

22) Balint S, Rao S, Sanchez M, Huntosova V, Miskovsky P, Petrov D, : Diffusion and cellular uptake of drugs in live cells studied with surface-enhanced Raman scattering probes; J. Biomedical Optics; 15 (2): 027005 (2010)

23) Kascakova S, Nadova Z, Mateasik A, Mikes J, Huntosova V, Refregiers M, Sureau F, Maurizot JC, Miskovsky P, Jancura D, : High- level of low-density lipoprotein receptors enhance Hyp uptake by U-87 MG cells in the presence of LDL; Photochem Photobiol. ; 84 (1): 120-127 (2008)

Proceedings:
24) Buzova D, Huntosova V, Kasak P, Petrovajova D, Joniova J, Dzurova L, Nadova Z, Sureau F, Miskovsky P, Jancura D.: Towards increased selectivity of drug delivery to cancer cells: development of a LDL-based nanodelivery system for hydrophobic photosensitizers; Proc. SPIE 8460, Biosensing and Nanomedicine V, 84600U (2012)

25) Jurasekova Z., Huntosova V., Belej D., Miskovsky P., Jancura S.: Human serum albumin-[Ru(Phen)3]2+complex formation studied by optical spectroscopies; Biophysical Journal-10.1016/j.bpj.2015.11.325, 110:48A-48A (2016)

26) Gerelli E., Huntosova V., Horvath D., Wagnieres G.: In vivo measurement of the tissue oxygenation by time-resolved luminescence spectroscopy of protoporphytin IX: Strategies to minimize artefacts associated with photoproducts; Proc. SPIE 10060:1006016, Optical Biopsy XV: Toward real-time spectroscopic imaging and diagnosis. (2017)

L’ORÉAL-UNESCO for women in Science an honorable mention for project entitled: Targeted anti-cancer drug delivery to breast cancer (2018)

Slovak Biophysical Society’s young scientist award (under 35 years old) for the scientific work in the field of Biophysics in years 2014, 2015. (April 2016)

IUPAC (International Union of Pure and Applied Chemistry) award for the best poster presentation at 16th International Congress on Photobiology, (September 2014), Cordoba, Argentina. Poster presentation: Effect of ROS created during pO2 detection by [Ru(Phen)3]2+ luminescence lifetime measurements

CSEM award for the best oral or poster presentation by young researcher at the yearly meeting of the Biomedical Photonics Network: Progress in Biomedical Photonics, (November 2011), Neuchatel, Switzerland. Poster presentation: Assessment of the tissue oxygenation in vivo by measuring the luminescence lifetime of Ru(Phen)32+ as poorly photosensitizing probe

The Slovak-Czech woman foundation for young women in science
The fellowship of Ľudmila Čuchranova for attending the 13th Congress of European Society for Photobiology in conjunction with the „nd congerence of the European Platform fro Photodynamic Medicine, 5-10 September 2009, Wroclaw, Poland.

The fellowship of Socrates/Erasmus program
The fellowship for 3 months stay at theUniversity of Pierre and Marie Curie in Paris, France. April-June 2008

The European Society for Photobiology (ESP)
The fellowship for attending the 34th Meeting of the American Society for Photobiology (ASP) in Burlingame, California, USA, 20-25 June 2008

The fellowship for attending the 12th Congress of ESP in Bath, UK, 1-6 September 2007

Honourable mention of the president of Faculty of Science, Pavol Jozef Safarik University in Košice for the scientific work (2006).

Slovak literary found award for the excellent student scientific work entitled: Spectroscopic study of influence HSA and LDL to accumulation and distribution hypericin in cell U-87 MG (2006)
Diploma of the 1st place in the Biophysical and Biomedical section at the National Student Scientific Conference in Bratislava (2006).

Diploma of the 2nd place in Biochemical section at Student Scientific Conference in Košice (2006).