doc., RNDr. Erik Sedlák, PhD.

Associate professor at Department of Chemistry, Faculty of Natural Sciences, Safarik University in Kosice (UPJS), PhD. degree in Biochemistry (1998), UPJS, associate professor in Chemistry at UPJS from 2006. Specialist in protein chemistry and enzymology. Recent research interests: structure and stability of biomacromolecules and their complexes with low molecular ligands; thermodynamics and kinetics stability of proteins such as IgGs, DARPins, GOX, LOV domain, and membrane proteins; Hofmeister effect on properties of enzymes, particularly proteases.

Education:
1976 – 84 Elementary school, ZS Gottwaldova/ZS Studentska Snina
1984 – 88 High school, Gymnázium Snina
1988 – 93 Safarik University Kosice (Slovakia) – MSc. in Biophysics
1993 – 97 Safarik University Kosice – Ph.D. study in Biochemistry
1998 – UPJS – Defence of Ph.D. thesis
2006 – Habilitation to Associate Professor of chemistry at Department of Biochemistry PF UPJS

Languages
English, Slovak

Professional positions:
1996–1998 Research Associate in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
1999–2001 Research Scientist & Faculty Member in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2002–2007 Research Scientist & Faculty Member in Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2003–2007 Head of Department of Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2007–2013 Several continual foreign stays
2010–2011 Associate Editor for Biochemistry in Chemical papers (Bratislava)
2011–2013 Editor in Chemical papers (Bratislava)
2013-presence Senior Research Associate at Centre for Interdisciplinary Biosciences, P.J. Šafárik University, Košice, Slovak Republic, Associate Professor at Department of Biochemistry, , P.J. Šafárik University, Košice, Slovak Republic
2018-presence Head of Center for Interdisciplinary Biosciences, Technology and Innovation Park, P.J. Šafárik University, Košice, Slovak Republic

Postdoctoral research training:
1994 (1 month), 1995 (3 months), 1997 (1 month), 2000 (2 months), 2002 (1 month) 2004 (1 month), 2005 (1 month) Laboratorium für Biochemie, Universität Bayreuth, Germany, with Prof. Dr. Mathias Sprinzl, Dr.h.c.
1998–1999 (21 months) Postdoctoral Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof. Neal C. Robinson. Project: „Functional Association of Cardiolipin with Cytochrome c Oxidase and bc1.“
2001–2002 (12 months) Postdoctoral Senior Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof.
2003 (3 months) Neal C. Robinson. Project: „Function of Cardiolipin in Cytochrome c Oxidase and bc1.“
2007–2008 (18 months) Postdoctoral Senior Research Associate in Biochemistry, The Rice University, Houston, USA, with Assoc. Prof. Dr. Pernilla Wittung-Stafshede. Project: „Folding mechanisms of copper transport proteins.“
2008–2009 (12 months) Postdoctoral Senior Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof. Neal C. Robinson. Project: “The functional and structural role of phospholipids bound to mitochondrial complexes.”
2009–2013 (46 months) Postdoctoral Associate in Biochemistry, The University of Zürich, Zürich, Switzerland with Prof. Dr. Andeas Plückthun. Projekt: “ Conversion of integral membrane receptors into soluble forms ” (Marie-Curie Intra-European Fellowship)
2014 (4 months) Visiting Researcher in laboratory of Prof. Dr. Andeas Plückthun, Department of Biochemistry, The University of Zürich, Zürich, Switzerland
2016 (2 months) Visiting Researcher in laboratory of Prof. Dr. Andeas Plückthun, Department of Biochemistry, The University of Zürich, Zürich, Switzerland

Honor and awards
• The best scientific work or collection of works of young scientist (under 35 years) in biophysics (granted by Slovak Biophysical Society; 2004)
• Award of Minister of Education of Slovak Republic for Science and Technique in category of Established results of young workers in science and development to 35 years; 2004.
• Reception by President of Slovak Republic; January 19th, 2005.
• Marie-Curie Intra-European Fellowship (~200,000 Eur) (2009–2011) in laboratory of Prof. Dr. Andreas Plückthun, University of Zürich. Project: “ Conversion of integral membrane receptors into soluble forms

Technical expertise:
Technical Expertise:

(i) Ribosome and Yeast displays – directed evolution methods for peptides/proteins.
(ii) Spectral techniques – absorbance spectroscopy, fluorescence and circular dichroism.
(iii) Differential scanning calorimetry – determination of thermodynamic parameters from microcalorimetric measurements of reversible and irreversible transitions; isothermal titration calorimetry.
(iv) Chromatography and analytical techniques (HPLC, FPLC), ELISA, Western blotting, SDS PAGE, with experience in analysis of experiments on analytical ultracentrifugation (from Center for Analytical Ultracentrifugation of Macromolecular Assemblies (CAUMA) in San Antonio).
(v) Protein purification (from heart tissue – cytochrome c oxidase, from bacteria – translation, elongation factors, heterologously expressed proteins).
(vi) Basic molecular biology, biochemical and biophysical methods.

Scientific projects (E.S. principal or co-principal investigator):

I. Science grant agency of the Ministry of education of the SR and of the Slovak Academy of Sciences (VEGA) (6)
2017–2019 VEGA 2/0009/17 (co-principal investigator)
Project: Oxidative stress and phospholipid-protein interactions: functional and structural consequences
2014–2016 VEGA 2/0062/14 (11 388 €, co-principal investigator)
Project: Functionality and Structural Integrity of Proteins in Bicelles – Implications for Mitochondrial and Amyloidogenic Proteins
2012–2014 VEGA 1/0521/12 (6 225 €, co-principal investigator)
Project: Modulation of protein dynamics and catalytical properties of enzymes by
Hofmeister ions.
2007–2009 VEGA 1/4319/07 (18 787 €, co-principal investigator)
Project: Study of conformational, functional, and sequentional diversities of
flavoproteins and chosen homodimeric oxidases.
2006–2008 VEGA 1/3252/06 (17 991 €, principal investigator)
Project: Stability and dynamics of chosen multidomain translational factors from
thermophile bacteria Thermus thermophilus.
2004–2006 VEGA 1/1272/04 (18 190 €, co-principal investigator)
Project: Effect of polyanions on structure, stabilty, and solubility of proteins.
2003–2005 VEGA 1/0432/03 (22 737 €, principal investigator)
Project: Modulation of structural and functional properties of flavoproteinoxidases
from thermophile Thermus thermophilus and mesophile Aspergillus niger by
modification of solvent properties.
II. Slovak Research and Development Agency (APVV) (1)
2016–2020 APVV 15–0069 (170 000 €, principal investigator)
Project: Conversion of integral membrane protein into water soluble form: the case of GPCR
III. Other grants:
2009–2011 Marie-Curie Intra-European Fellowship (~200 000 €, principal investigator)
2005 Deutsche Forschungsgemeinschaft in collaboration with Prof.Dr. Mathias Sprinzl, Dr.h.c., Universität Bayreuth, Germany (12 613 €, co-principal investigator)
2005 Development project awarded by Ministry of education of Slovak Republic (17 426 €, principal investigator)

Invited congress lectures:
7 lectures (international congresses) including 3 plenary lectures
Graduated PhD students:
• 2014 Kamil Tóth (thesis defense: UPJS)
• 2011 Nataša Tomášková (thesis defense: UPJS)
• 2006 Gabriel Žoldák (as co-supervisor, thesis defense: UPJS)
Diploma students:
15 finished, 2 in the process.
Publications:
63 publications in international peer-reviewed journals (see WOS and Scopus databases), cited more than 780 times, Hirsch index 19
Kosice, Januar, 2018