doc. RNDr. Daniel Jancura, PhD.

 

Education:

1988 – 93 MSc. in Biophysics, Safarik University in Kosice (Slovakia)
1993 – 97 PhD study in Biophysics, Safarik University in Kosice
1999 Defense of PhD thesis, Comenius University in Bratislava (Slovakia)
2009 Habilitation to Associate Professor, Safarik University in Kosice

 

Research interest:

– targeted drug delivery – construction and characterization of lipoprotein based drug delivery systems
– catalytical mechanism of cytochrome c oxidase
– application of surface-enhanced Raman spectroscopy (SERS) for detection of low concentrations of drugs and pollutants

 

Head of the Department of Biophysics, Faculty of Natural Science, Safarik University in Kosice, Slovakia (UPJS), President of the Slovak Biophysical Society, PhD. degree in Biophysics (1999), Comenius University in Bratislava (Slovakia), associate professor in Biophysics at UPJS from 2009. Specialist in molecular biophysics and molecular bioenergetics, recent research interests: structure and stability of biomacromolecules and their complexes with low molecular ligands, construction and characterization of lipoprotein based drug delivery systems, catalytical mechanism of cytochrome c oxidase.

Education:

1976 – 84 Elementary school, ZS Hrnčiarska Humenné
1984- 88 High school, Gymnázium Humenné
1988 – 93 Safarik University Kosice (Slovakia)-MSc. in Biophysics
1993 – 97 Safarik University Kosice – PhD study in Biophysics
1999 Comenius University Bratislava (Slovakia) – Defence of PhD thesis

Professional positions:

1997 – 1999 Assistant professor, Department of Biophysics, Safarik University Kosice
2002 – 2009 Assistant professor, Department of Biophysics, Safarik University Kosice
2009 – Associate profesor, Department of Biophysics, Safarik University, Kosice
2015 – Head of the Department of Biophysics, Safarik University, Kosice

Doctorate and postdoctoral research training and foreign stays:

1993 Dipartimento de Biochimica, Universita Bologna (Italy)
1996 – 1997 Instituto de Estructura de la Materia, CSIC , Madrid (Spain)
1999 – 2002 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2005 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2010 Department of Biochemistry & Cell biology, Rice University, Houston (USA)
2011 Department of Biochemistry & Cell biology, Rice University, Houston (USA)

Professional orientation:

Targeted drug delivery – construction and characterization of lipoprotein based drug delivery systems, catalytical mechanism of cytochrome c oxidase

Hobby:

Sport (football, tenis, basketball)
Literature (scientific literature, politics, economy, Music (60‘s)

International projects (5):

Grant agency and project number: R.A. Welch Foundation Grant C-636
Title: Catalytic mechanism of heme-copper oxidases
Duration: 1999-2002
Budget: 400 000 $
Member of the scientific team

Grant agency and project number: NIH GM 55807
Title: Catalytic mechanism of heme-copper oxidases
Duration: 2002-2005
Budget: 1 000 000 $
Member of the scientific team

Grant agency and project number: COST Action FAO602
Title: Bioactive food components, mitochondrial function and health
Duration: 2008-2011
Budget: 100 000 EURO
Member of the Management Committee

Gran agency and project number: EU 7. FP-REGPOT, CELIM 316310
Title: Fostering excellence in multiscale cell imaging (CELIM)
Duration: 2013-2016
Budget: 2 700 000 EURO
Member of the scientific team and coordinator of the Activity I – Recruitment

Grant agency and project number: COST Action CA15126
Title: Between atom and cell. Integrating molecular biophysics approaches for biologfy and healthcare (MOBIEU)
Duration: 2016-2020
Budget: 530 000 EURO
Member of the Management Committee)

National projects:

Slovak Research and Development Agency (APVV) (6):

Grant agency and project number: APVV 0485/15
Title: Towards highly selective cancer treatment: Endogenous lipoprotein-DARPin complexes as a new generation of targeted drug delivery vehicles (DARLINK)
Duration: 2016-2020
Budget: 247 000 EURO
Principal investigator and coordinator of the Activity I – Construction and optimization of lipoprotein-based drug delivery vehicle

Grant agency and project number: APVV 0069/15
Title: Conversion of integral membrane protein into water soluble form: the case of GPCR
Duration: 2016-2020
Budget: 184 000 EURO
Member of the scientific team

Grant agency and project number: APVV 0242/11
Title: Towards increased sensitivity of cancer detection and selectivity of cancer treatment biophotonic nanotechnology applications (NanoTechPDT)
Duration: 2011-2015
Budget: 172 000 EURO
Member of the scientific team and coordinator of the Activity 2 – Nanoparticle transport systems for targeted and effective drug delivery

Grant agency and project number: APVV 0449/07
Title: Targeted photodynamic therapy of cancer: from delivery system through cellular signaling to in vivo model
Duration: 2008-2010
Budget: 200 000 EURO
Member of the scientific team and coordinator of the Activity 1 – Physico-chemical characterization of low-density lipoprotein – based delivery systems loaded with photosensitizers hypericin and Foscan

Grant agency and project number: APVV 0361/04
Title: Photodynamic therapy of cancer in the light of new molecular knowledge: mechanism of antitumor action of naturally occuring hydroxyquinone photosensitizers
Duration: 2005-2007
Budget: 136 000 EURO
Member of the scientific team

Grant agency and project number: APVV 0222/02
Title: New physical and chemical approaches to photodynamic therapy of cancer
Duration: 2002-2004
Budget: 98 000 EURO
Member of the scientific team

Scientific grant Agency of The Ministry of Education and Science of the Slovak Republic (VEGA) (6):

Grant agency and project number: VEGA 0464/18
Title: Coupling of electron-proton transfer in respiratory heme-copper oxidases
Duration: 2018 – 2022
Budget: 52 000 EURO
Co-principal investigator

Grantová agentúra a číslo projektu : VEGA 0164/09
Názov projektu : Photochemical, photophysical and biophysical characterization of low-density lipoproteins based delivery systemsloaded witk photosensitiyers hypericin and Foscan
Doba trvania: 2009-2011
Celkový rozpočet: 16 000 EURO
Vedúci projektu

Grantová agentúra a číslo projektu : VEGA 4449/07
Názov projektu : Detection of trace amounts of chemicals compounds by chemical sensors based on roughened metalic surfaces
Doba trvania: 2007-2009
Celkový rozpočet: 5 000 EURO
Zástupca vedúceho projektu

Grantová agentúra a číslo projektu : VEGA 2278/05
Názov projektu : Interaction of natural photosensitizers hypericin and calphostin C with regulatory and transport proteins
Doba trvania: 2005-2007
Celkový rozpočet: 15 000 EURO
Zástupca vedúceho projektu

Grantová agentúra a číslo projektu : VEGA 9040/02
Názov projektu : Antiviral and antitumor activity of natural photoactive molecules hypericin and hypocrellin: interaction with biological macromolecules and mechanism of action at cellular and molecular levels
Doba trvania: 2002-2004
Celkový rozpočet: 11 000 EURO
Člen riešiteľského kolektívu

Grantová agentúra a číslo projektu : VEGA 3258/96
Názov projektu : Antiviral activity of aromatic polycyclic diones:mechanism of interaction on cellular and molecular levels
Doba trvania: 1996-1998
Celkový rozpočet: 10 000 EURO
Člen riešiteľského kolektívu

Projekty zo štrukturálnych fondov EÚ:

SEPO I, II
CEVA II
NanoBioSens
Doktorand

Number of articles in CC journals : 53
Number of SCI citations : 410
H – index: 16

Contributions in monography:

1. P. Sevilla, G. Lajos, R. de Llanos, S. Sanchez-Cortes, D. Jancura, P. Miskovsky and J.V Garcia-Ramos. Surface – enhanced fluorescence and Raman spectroscopy (SEF and
SERS) of anthraquinone anti-tumoral drugs and their complexes with biomolecules on Ag nanoparticles, in Advances in Biomedical Spectroscopy 5, Applications of Raman
spectrosscopy to Biology: From basc studies to disease diagnosis (ed. M. Ghomi), pp, 31-57, IOS Press, 2012.

List of CC publications:

1. G. Fabriciová, P. Miškovský, D.Jancura, E. Kočišová and V. Lisý. Conformational transitions of poly ( dI-dC) as studied by classical Raman spectroscopy. Gen. Physiol. Biophys. 14, 203 216 (1995).

2. D.Jancura, S. Sánchez-Cortés, E. Kočišová, A Tinti, P. Miškovský and A. Bertoluzza. Surface-enhanced resonance Raman spectroscopy of hypericin and emodin on silver colloids. SERRS and NIR FTSERS study. Biospectroscopy 1, 265 273 (1995).

3. S. Sánchez-Cortes, P. Miškovský, D. Jancura and A. Bertoluzza. Specific interactions of antiretroviraly active drug hypericin with DNAas studied by surface-enhanced resonance Raman spectroscopy. J. Phys. Chem. 100, 1938-1944 (1996).

4. S. Sánchez-Cortés, D. Jancura, P. Miškovský and A. Bertoluzza. Near infrared surface- enhanced Raman spectroscopic study of antiretroviraly drug hypericin and emodin in aqueous silver colloids. Spectrochimica Acta Part A 53, 769-779 (1997).

5. G. Wheeler, P. Miškovský, D. Jancura and L. Chinsky. A study of metalloporphyrin polynucleotide interactions by microcalorimetry and circular dichroism. J. Biomol. Str. Dyn. 15, 967-985 (1998).

6. P. Miškovský, D. Jancura, S. Sánchez-Cortés, E. Kočišová and L. Chinsky. Antiretroviraly active drug hypericin binds the IIA subdomain of human serum almumin. Resonance Raman and surface-enhanced Raman spectroscopy study. J.Am. Chem. Soc. 120, 6374-6379 (1998).

7. D. Jancura, S. Sánchez-Cortes, P. Miškovský, L. Rivas, J. Staničová and J.V. García-Ramos. Surface-enhanced resonace Raman spectroscopy of hypocrellin A An effect of excitation wavelength and pH . Spectrochimica Acta Part A 54, 1519-1526 (1998).

8. G. Fabriciová, P. Miškovský, D. Jancura and V. Lisý. Charcterization of low salt and high salt conformation of poly (dI-dC) by hydrogen-deuterium exchange kinetics A classical Raman spectroscopy study. J. Biomol. Str. Dyn. 16, 281-288 (1998).

9. G. Fabriciová, P. Miškovský, D. Jancura, V. Lisý and L. Chinsky. Conformatiponal study of poly (dI-dC): Hydrogen-deuterium exchange and low frequency resonance Raman spectroscopy. J. Mol. Str. 480-481, 449-453 (1999).

10. E. Kočišová, D. Jancura, S. Sánchez-Cortés, P. Miškovský, L. Chinsky and J.V. García- Ramos. Interaction of antiviral and antitumoral photoactive drug hypocrellin A with human serum albumin. J. Biomol. Str. Dyn. 17, 111-120 (1999).

11. M. Fabian, D. Jancura and G. Palmer. Two sites of interaction of anions with cytocrome a in oxidized bovine cytochrome c oxidase. J. Biol. Chem. 279, 16170-16177 (2004).

12. M. Fabian, L. Skultety, D. Jancura and G. Palmer. Implications of ligand binding studies for the catalytic mechamism of cytochrome c oxidase. BBA-Bioenergetics 1655,298-305 (2004).

13. J.Hritz, J. Ulicny, A. Laaksonen, D. Jancura and P. Miskovsky. Molecular interaction model for the C1B domain of protein kinase C in the complex with its activator phorbol 12-myristate 13- acetate in water solution and lipid bilayer. J.Med.Chem. 47, 6547-6555 (2004).

14. M. Antalik, D. Jancura, G. Palmer and M. Fabian. A role for the protein in internal electron transfer to the catalytic center of cytochrome c oxidase. Biochemistry 44, 14881-14889 (2005).

15. S. Kascakova, M.Refregiers, D. Jancura, F. Sureau, J.C. Maurizot and P. Miskovsky. Fluorescence spectroscopic study of hypericin-photosensitized oxidation of low-density lipoproteins. Photochem.Photobiol. 81, 1395-1403 (2005).

16. M. Fabian, D. Jancura, M. Bona, A. Musatov, M. Baran and G. Palmer. Influence of reduction of heme a and CuA on the oxidized catalytic center of cytochrome c oxidase: Insight from organic solvents. Biochemistry 45, 4277-4283 (2006).

17. S. Kocanova, T. Hornakova, J. Hritz, D. Jancura, D. Chorvat jr., A. Mateasik, J. Ulicny, M. Refregiers, J.C. Maurizot and P. Miskovsky. Characterization of the interaction of hypericin witk protein kinase C in U-87 MG human glioma cells, Photochem. Photobiol. 82, 720-728 (2006).

18. D. Jancura, M. Antalik, V. Berka, G. Palmer and M. Fabian. Filling the catalytic site of cytochrome c oxidase with electrons : Reduced CuB facilitates internal electron transfer to heme a3. J. Biol. Chem. 281, 20003-20010 (2006).

19. D. Jancura, V. Berka, M. Antalik, J. Bagelova, R.B. Gennis, G. Palmer and M. Fabian. Spectral and kinetic equivalence of oxidized cytochrome c oxidase as isolated and „activated“ by reoxidation. J. Biol. Chem. 281, 30319-30325 (2006).

20. M. Savko, S. Kascakova, P. Mojzes, D. Jancura, P. Miskovsky and J. Ulicny. Ground and excited state properties of naphthazarin: Absorption spectroscopy and theoretical modeling study, J.Mol.Struct. THEOCHEM 803, 79-87 (2007).

21. S. Kascakova, Z. Nadova, A. Mateasik, J. Mikes, V. Huntosova, M. Refregiers, F. Sureau, J.C. Maurizot, P. Miskovsky and D. Jancura. High level of low-density lipoprotein receptors enhance hypericin uptake by U-87 MG cells in the presence of LDL. Photochem. Photobiol. 84, 120-127 (2008).

22. P. Gbur, R. Dedic, D. Jancura, P. Miskovsky and J. Hala. Time resolved luminescence and singlet oxygen formation under illumination of hypericin in acetone. Journal of Luminescence, 128, 765-767 (2008),

23. G.Lajos, D.Jancura, P.Miskovsky, J.V.Garcia-Ramos and S.Sanchez-Cortes. Surface-enhanced fluorescence and Raman scattering study of antitumoral drug hypericin: An effect of aggregation and self-spacing depending on pH. J.Phys.Chem. C 112, 12974-12980 (2008).

24. P. Gbur, R. Dedic, D. Chorvat jr., P. Miskovsky, J. Hala and D. Jancura. Time resolved luminescence and singlet oxygen formation after illumination of hypericin-low-density lipoproteins complex. Photochem. Photobiol. 85, 816-8223 (2009).

25. G. Lajos, D. Jancura, P. Miskovsky, J.V. Garcia-Ramos and S. Sanchez-Cortes. Interaction of the photosensitizer hypericin with low-density lipoproteins and phosphatidylcholine: A surface – enhanced Raman scattering and surface-enhanced fluorescence study. J. Phys. Chem. C 113, 7147-7154 (2009).

26. V. Huntosova, L. Alvarez, L. Bryndzova, Z. Nadova, D. Jancura, L. Buriankova, S. Bonneau, D. Brault, P. Miskovsky and F. Sureau. Interaction dynamics of hypericin with low-density lipoproteins and U-87-MG cells. Int. J. Pharmaceutics 389, 32-40 (2010).

27. L. Buriankova, Z. Nadova, D. Jancura, M. Refregiers, I. Yousef, J. Mikes and P. Miskovsky. Synchrotron based Fourier-transform infrared microspectroscopy as sensitive technique for the detection of early apoptosis in U-87 MG cells. Laser Phys. Lett. 7, 613-620 (2010).

28. L. Buriankova, D. Buzova, D. Chorvat jr., F. Sureau, D. Brault, P. Miskovsky and D. Jancura. Kinetics of hypericin association with low-density lipoproteins. Photochem. Photobiol. 87, 56-63 (2011).

29. G. Bano, J. Stanicova, D. Jancura, J. Marek, M. Bano, J. Ulicny, A. Strejckova and P. Miskovsky. On the diffusion of hypericin in dimethylsulfoxide/water mixtures-The effect of aggregation. J. Phys. Chem. B115, 2417-2423 (2011).

30. V. Huntosova, D. Buzova, D. Petrovajova, P. Kasak, Z. Nadova, D. Jancura, F. Sureau and P. Miskovsky. Development of a new LDL-based transport system for hydrophobic/amphiphilic drug delivery to cancer cells. Int. J. Pharmaceutics 436, 463-471 (2012).

31. B. Buzova, V. Huntosova, P. Kasak, D. Petrovajova, J. Joniova, L. Dzurova, Z. Nadova, F. Sureau, P. Miskovsky and D. Jancura. Towards increased selectivity of drug delivery to cancer cells: development of a new LDL-based nanodelivery system for hydrophobic photosenzitizers. Proceedings of SPIE vol. 8460, Biosensing and Nanomedicine V, 84600U (2012).

32. A. Strejckova, J. Stanicova, D. Jancura, P. Miskovsky and G. Bano. Spatial orientation and electric-field-driven transport of hypericin inside of bilayer lipid membranes. J. Phys. Chem. B 117, 1280-1286 (2013).

33. E. Lopez-Tobar, M. Antalik, D. Jancura, M.V. Canamares, A. Garcia-Leis, D. Fedunova, G. Fabriciova and S. Sanchez-Cortes. Adsorption and detection of amyloid marker thioflavin T on Ag nanoparticles by surface-enhanced Raman scattering. J. Phys. Chem. C 117, 3996-4005 (2013).

34. D. Petrovajova, D. Jancura, P. Miskovsky, D. Chorvat jr., A. Chorvatova, X. Ragas, M. Garcia-Diaz, S. Nonell and Z. Nadova. Monitoring of singlet oxygen luminescence and mitochondrial autofluorescence after illumination of hypericin/mitochondria complex: a time-resolved study. Laser Physics Letters 10, art. no. 075609 (2013).

35. D. Buzova, P. Kasak, P. Miskovsky and D. Jancura. Solubilization of poorly soluble photosensitizer hypericin by polymeric micelles and polyethylene glycol. Gen. Phys. Bioph. 32, 201-208 (2013).

36. J. Kubackova, I. Izquierdo-Lorenzo, D. Jancura, P. Miskovsky and S. Sanchez-Cortes. Adsorption of linear aliphatic α,ω-dithiols on plasmonic metal nanoparticles: a structural study based on surface-enhanced Raman spectra. Phys. Chem. Chem. Phys. 16, 11461-11470 (2014).

37. D. Jancura, J. Stanicova, G. Palmer and M. Fabian. How hydrogen peroxide is metabolized by oxidized cytochrome c oxidase. Biochemistry 53, 3564-3575 (2014).

38. J. Joniova, L. Buriankova, D. Buzova, P. Miskovsky and D. Jancura. Kinetics of incorporation/redistribution of photosensitizer hypericin to/from high-density lipoproteins. Int. J. Pharmaceutics 475, 578-584 (2014).

39. J. Joniova, L. Blascakova, D. Jancura, Z. Nadova, F. Sureau and P. Miskovsky. Incorporation of photosensitizer hypericin into synthetic lipid-based nanoparticles for drug delivery and large unilamellar vesicles with different content of cholesterol. Proceedings of SPIE vol. 9166, Biosensing and Nanomedicine VII, 911604 (2014).

40. J. Varchola, V. Huntosova, D. Jancura, G. Wagnieres, P. Miskovsky and G. Bano. Temperature and oxygen-concentration dependence of singlet oxygen production by RuPhen as induced by quasi-continuous excitation. Photochem. Photobiol. Sci. 13, 1781-1787 (2014).

41. J. Kubackova, G. Fabriciova, P. Miskovsky, D. Jancura and S. Sanchez-Cortes. Sensitive surface-enhanced Raman spectroscopy (SERS) detection of organochlorine pesticides by alkyl dithiol-functionalized metal nanoparticles induced plasmonic hot spots. Anal. Chem. 87, 663-669 (2015).

42. E. Lopez-Tobar, V. Verebova, L. Blascakova, D. Jancura, G. Fabriciova and S. Sanchez-Cortes. Detection and aggregation of the antitumoral drug parietin in ethanol/water mixture and on plasmonic metal nanopartcles studied by surface-enhanced optical spectroscopy: Effect of pH and ethanol concentration. Spectrochimica Acta Part A. Mol. Biomol. Spectr. 159, 134-140 (2016).

43. J. Varchola, K. Zelonkova, D. Chorvat jr., D. Jancura, P. Miskovsky and G. Bano. Singlet oxygen produced by quasi-continuous photo-excitation of hyperiin in dimethyl sulfoxide. J. Luminescence 177, 17-21 (2016).

44. A. Garcia-Leis, D. Jancura, M. Antalik, J.V. Garcia-Ramos, S. Sanchez-Cortes and Z. Jurasekova. Catalytic effects of silver plasmonic nanoparticles on the redox reaction leading to ABTS.+ formation studied using UV- visible and Raman spectroscopy. Phys. Chem. Chem. Phys. 18, 26562-26571 (2016).

45. J. Joniova, M. Rebic, A. Strejckova, V. Huntosova, J. Stanicova, D. Jancura, P. Miskovsky and G. Bano. Formation of large hypericin aggregates in giant unilamellar vesicles – experiments and modeling. Biophys. Journal 112, 966-975 (2017).

46. G. Zoldak, D. Jancura and E. Sedlak. The fluorescence intensities ratio is not a reliable parameter for evaluation of protein unfolding transitions. Protein Science 26, 1236-1239 (2017).

47. D. Belej, Z. Jurasekova, M. Nemergut, G. Wagnieres, D. Jancura and V. Huntosova. Negligible interaction of Ru(Phen)2 2+ with human serum albumin makes it promising for a reliable in vivo assessment of the tissue oxygenation. J. Inorg. Biochem. 174, 37-44 (2017).

48. P. Kesa, D. Jancura, J. Kudlacova, E. Valusova and M. Antalik. Excitation of triplet states of hypericin in water mediated by hydrtropic cromolyn sodium salt. Spectrochimica Acta Part A. Mol. Biomol. Spectr.193, 185-191 (2018).

49. A. Hovan, S. Datta, S.G. Kruglik, D.Jancura, P. Miskovsky and G. Bano. Phosphorescence kinetics of singlet oxygen produced by photosensitization in spherical nanoparticles. Part I. Theory. J. Phys. Chem. B 122, 5147-5153 (2018).

50. S. Datta, A. Hovan, A. Jutkova, S.G. Kruglik, D.Jancura, P. Miskovsky and G. Bano. Phosphorescence kinetics of singlet oxygen produced by photosensitization in spherical nanoparticles. Part II. The case of hypericin-loaded low-density lipoprotein particles. J. Phys. Chem. B 122, 5154-5160 (2018).

51. S. Datta, A. Jutkova, P. Sramkova, L. Lenkavska, V. Huntosova, D. Chorvat, P. Miskovsky, D. Jancura and J. Kronek. Unravelling the excellent chemical stability and bioavailability of solvent responsive curcumin-loaded 2-ethyl-2 oxazoline-grad-2-(4-dodecyloxyphenyl)-2-oxazoline copolymer nanoparticles for drug delivery. Biomacromolecules 19, 2459-2471 (2018).

52. K. Kopcova, L. Blascakova, T. Kozar, D. Jancura and M. Fabian. Response of heme symmetry to the redox state of bovine cytochrome c oxidase. Biochemistry 57, 4105-4113 (2018).

53. L. Blascakova, D. Horvath, D. Belej, G. Wagnieres, P. Miskovsky, D. Jancura and V, Huntosova. Hypericin can cross barriers in the chickens chorioallantoic membrane model when delivered in low-density lipoproteins. Photodiagnosis Photodynamic Therapy 23, 306-313 (2018).

President of the Slovak Biophysical Society (2016-)
Member of the Steering committees of two COST projects
Member of the start-up SAFTRA Photonics – winner of the “Start-up Awards 2017” in Slovakia